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This volume details protocols in Adipogensis research, such as stem cell line isolation, gene expression, obesity research, and drug development. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Adipogenesis: Methods and Protocols aims to be comprehensive guide for researchers in the field.
List of contents
Adipogenic differentiation from human embryonic stem cells through formation of embryoid bodies.- Quantification of adipogenesis using flow cytometry.- Isolation of Wharton s Jelly derived Mesenchymal Stromal/Stem Cells.- Adipogenic differentiation assay for the study of insulin signalling.- Quantification of lipid droplets in adipocytes.- RT-qPCR quantification of adipogenic marker genes (Pparg, C/ebpa, Fabp4 and Cd36).- Adipogenic differentiation of human adipose-derived mesenchymal stromal/stem cells (ASCs).- Western blot detection of adipogenic protein.- Measurement of intracellular Reactive Oxygen Species during adipogenesis.- Oxygen consumption and extra cellular acidification rate in adipogenic differentiated human mesenchymal stromal/stem cells.- In vitro expansion of human adipose-derived stem cells (ASCs).- siRNA silencing of adipogenic gene in ASCs.- Freezing and thawing of Adipose-derived stromal/stem cells (ASCs).- Isolation and Characterization of Mouse Adipose-Derived Stromal/Stem Cells.- Isolation of adipose-derived mesenchymal stromal/stem cells.- Isolation and purification of adipocyte-derived extracellular vesicles (AdEVs) using size-exclusion chromatography.- Differentiation of Adipose derived stromal/stem cells into Osteocytes.- Differentiation of mesenchymal stromal/stem cells from induced pluripotent stem cells.- Isolation and Characterization of Equine Mezenchymal Stromal/Stem Cell.
