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This collection explores advances in genome sequencing and analysis tools for the understanding of insect genomes and hence their biology. Beginning with obtaining a high-quality genome assembly using the latest sequencing data, the book continues with the identification of genome variability including single nucleotide polymorphisms and genomic inversions, and the annotation of insect genomes through genes, transposable elements, and piRNA clusters. Notably, chapters also delve into downstream analyses, such as the evolution of gene families and phylogenetics, molecular biology protocols pertinent to the study of insects, as well as CRISPR-era genome editing tools to manipulate non-model insects. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detailed implementation advice to ensure success in the laboratory.
Authoritative and practical, Insect Genomics: Methods and Protocols serves as an ideal source for state-of-the-art methods and comprehensive reviews to help researchers design and implement their own studies.
List of contents
De Novo Genome Assembly Using Long Reads and Chromosome Conformation Capture.- Identification of Single Nucleotide Polymorphism from Insect Genomic Data.- npstat: An Efficient Tool to Explore the Population Genome Variability and Divergence Using Pool Sequencing Data.- Navigating Eukaryotic Genome Annotation Pipelines: A Route Map to Using BRAKER, Galba, and TSEBRA.- A Concise Guide for the Characterization and Curation of Transposable Elements in Insect Genomes.- Annotation of piRNA Source Loci in the Genome of Non-Model Insects.- The Influence of Chromosomal Inversions on Genetic Variation and Clinal Patterns in Genomic Data of Drosophila melanogaster.- Evolutionary Genomics of Gene Families: A Case Study of Insect Gustatory Receptors.- Insect Phylogenomics: From Experiment Planning to Post-Phylogenetic Analyses.- High-Throughput Amplicon Sequencing for Analyzing Microbial Communities of Insects.- An Optimized SPLiT-Seq Protocol for Insects.- Identification of Sex-Specific and Sex-Biased Transcripts for Genetic Sexing.- Characterization of Mosquito Salivary Components.- History of Mosquito Transgenesis: A Perspective in Review.- Manipulation of a New Non-Model Insect Genome Using Targeted CRISPR-Era Approaches.- Adapting and Testing ReMOT Control for Expanded CRISPR-Era Genome Functions in Non-Model Insects.
Summary
Beginning with obtaining a high-quality genome assembly using the latest sequencing data, the book continues with the identification of genome variability including single nucleotide polymorphisms and genomic inversions, and the annotation of insect genomes through genes, transposable elements, and piRNA clusters.
