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This fully updated volume serves as multidisciplinary compendium of approaches and techniques employed to analyze the role of different molecules, processes, or strategies used by different guests to survive and proliferate in their associations with eukaryotic hosts. Beginning with animal-pathogen interactions, the book then continues with chapters exploring virus-host interactions, plant-microbe interactions, as well as different molecular techniques that were initially applied to non-pathogenic interactions but can be adapted to study other host-pathogen associations. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips for troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Host-Pathogen Interactions: Methods and Protocols, Second Edition contributes to the study of host-pathogen interactions with numerous techniques that can be used in a variety of bacteria.
List of contents
Host-Pathogen Interaction: Biology and Public Health.- Genetic Association Studies in Host-Pathogen Interaction Analysis.- Live-Cell Fluorescence Imaging for Virus/Host Interactions.- Construction of a Mycoviral Infectious Clone for Reverse Genetics in Botrytis cinerea.- Exopolysaccharide Production and Precipitation Method as a Tool to Study Virulence Factors.- Virulence-Related Assays for Investigation of the Acidovorax citrulli-Cucurbitaceae Pathosystem.- Dual-Fluorescence Chromosome-Located Labeling System for Accurate In Vivo Single-Cell Gene Expression Analysis in Pseudomonas syringae.- A Robust Method to Perform In Vitro and In Planta Interbacterial Competition Assays: Killing Plant Pathogens by a Potent Biocontrol Agent.- Quantification of Mixed-Linkage beta-Glucan (MLG) in Bacteria.- Surface Plasmon Resonance as a Tool to Elucidate the Molecular Determinants of Key Transcriptional Regulators Controlling Rhizobial Lifestyles.- Microscope SubcellularLocalization of Plant-Interacting Bacterial Effectors in Animal Cell Cultures.- A Workflow for the Functional Characterization of Noncoding RNAs in Legume Symbiotic Bacteria.- Methods for Studying Swimming and Surface Motilities in Rhizobia.- Isolation, Quantification, and Visualization of Extracellular Membrane Vesicles in Rhizobia Under Free-Living Conditions.- Isolation of Rhizobial Extracellular Membrane Vesicles from Bacteroids.- Nod Factor Lipopolysaccharide Purification to Study Nitrogen-Fixing Bacteria Symbiosis with Legumes.- A Novel System to Selective Tagging of Sinorhizobium fredii Symbiotic Plasmids.- New Inoculation Strategy for Legume Based on Rhizobium-Metabolite Co-Encapsulation.
