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Quantitative Gas Chromatography-Mass Spectrometry: Principles in Life Sciences and Biochemical Applications provides an in-depth exploration of the principles and practices of GC-MS. This comprehensive guide caters to researchers, academics, and graduate students engaged in analytical chemistry, offering both theoretical knowledge and practical applications. The book is structured to introduce the fundamentals before delving into advanced techniques, ensuring a thorough understanding for all readers. The book covers essential topics such as gas chromatography-tandem mass spectrometry, liquid chromatography-tandem mass spectrometry, and the use of stable isotopes and labeled analogs. It also presents practical advice on method development, validation, and quality control.
Additionally, it addresses common issues and troubleshooting in GC-MS, making it a valuable resource for those in fields like Biochemistry, Pharmacology, Forensic Medicine, and Metabolomics.
List of contents
1. Preface - instrumental quantitative analytical chemistry: GC-MS, GC-MS/MS; LC-MS, LC-MS/MS
2a. Principles of gas chromatography-mass spectrometry
2b. Principles of gas chromatography-tandem mass spectrometry
2c. Prinsiples of liquid chromatography-tandem mass spectrometry
3. Chemical ionization, electron ionization
4. Stable isotopologues as internal standards, standardization
5. Metabolic pathways - physiological substances, biomarkers, creatinine
6. Health and disease
7. Pharmacology
8a. Biochemical and clinical studies
8b. Pre-analytical and analytical issues
9. Extraction - solid, solvent, affinity chromatography
10. Chemical derivatization - methylation; PFBBr; PFPA
11. Quantitative analysis - accuracy, precision, LOD, LOQ
12. Method validation and comparison
13. Quality control
14. Isotope effects in chromatography
15. Biochemical and biomedical applications
15a. Nitrite, nitrate, creatinine, carbonate, malondialdehyde
15b. Prostaglandin, thromboxane, leukotrienes, fatty acids
15c. Amino acids
15d. Biogenic amines miscellaneous summary references
About the author
Dimitros Tsikas is a Professor at the Institute of Toxicology, Core Unit Proteomics in Hannover Medical School.
