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This book presents fundamental concepts in microbial biofilms, antimicrobial strategies, and examines various laboratory models to study biofilm formation. It also discusses analytical methods for studying biofilms. This book is useful for researchers, scientists, and students of microbiology.
List of contents
Chapter 1. Understanding microbial biofilms: characteristics, formation stages, and mechanisms of communication and immune system escape. Chapter 2. Biofilm extracellular matrix. Chapter 3. Mechanisms of antimicrobial resistance in biofilms. Chapter 4. Biofilms in the context of oral diseases. Chapter 5. Biofilms of medical significance in and around humans. Chapter 6. Development of novel antimicrobial therapeutic strategies for biofilm control. Chapter 7. Exploring the spectrum of biofilm complexity. Chapter 8. Laboratory models for biofilm studies. Chapter 9. Quantification of microbial biofilm biomass. Chapter 10. Quantification of cultivable biofilm cells: counting colony-forming units. Chapter 11. Analytical tools for assessing metabolic activity in biofilms. Chapter 12. Technical approaches to biofilm matrix analysis. Chapter 13. Omics techniques (genomics, transcriptomics, proteomics and metabolomics) in the study of oral and systemic biofilms. Chapter 14. Flow-cytometry analysis in biofilm research. Chapter 15. Imaging techniques in biofilm research. Chapter 16. Electrochemical detection of biofilms. Chapter 17. Machine learning approaches in biofilm research: An overview.
About the author
Dr. Ashutosh Kumar Shukla is a Professor of Physics at Ewing Christian College, Prayagraj, a constituent college of the University of Allahabad, India. His research interest is primarily based on the applications of spectroscopy techniques, including ESR spectroscopy, in different fields. Dr. Shukla has successfully completed research projects funded by the University Grants Commission, New Delhi, and presented his research findings at different international events. He has been awarded the bilateral exchange fellowship and visiting fellowship of the Indian National Science Academy, New Delhi, in addition to research fellowships from the Council of Science and Technology, Lucknow, and the Council of Scientific and Industrial Research, New Delhi. Dr. Shukla is on the review panel and editorial boards for international journals. With more than twenty-two years of teaching experience, he has to his credit journal publications, textbooks, and more than thirty edited volumes published by reputed international publishers.
Dr. Douglas Roberto Monteiro is an Assistant Professor at the Department of Diagnosis and Surgery, School of Dentistry, Araçatuba, São Paulo State University (UNESP), Brazil. He completed his doctoral degree (2012) and post-doctoral studies (2016) from the same institution, having developed part of his doctoral project at the Department of Biological Engineering of the University of Minho, Braga, Portugal (2010-2011). He has published over 85 research articles and 35 book chapters. He has also recently been listed as one of the top 2% of scientists, according to Stanford University (Elsevier BV 2021-2024), USA. So far, 7 MSc and 3 Ph.D. theses have been completed under his guidance. His research areas include microbial biofilms of interest in the medical and dental fields, fungal infections caused by
Candida species, alternative antimicrobial agents, and nanotechnology.
