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This volume covers the latest advancements and techniques used to understand the fastidious bacterium, Borrelia burgdorferi, and its significance in infectious disorders by combining both conventional and cutting-edge approaches. This book covers diverse topics, including direct detection, diagnostic methods, immune response analysis, alternative model systems, advanced proteomics, social media analysis, and clinical research. It also discusses unconventional wet lab research such as content analysis, the use of ChatGPT, clinical algorithms for chronic Lyme, establishment of a pregnancy Lyme disease biobank, and investigates Lyme in pregnant women. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Borrelia burgdorferi: Methods and Protocols encompasses a wide range of techniques and caters to scientists from various disciplines and career stages, such as cell and molecular biologists, statisticians, and clinical researchers.
List of contents
Real-Time Microfluidic PCRs: A High-Throughput Method to Detect 48 or 96 Tick-Borne Pathogens in 48 or 96 Samples.- Direct Detection of Borrelia Species in Tissues.- Purification of Borrelia burgdorferi Outer Membrane Vesicles.- Constructing an ELISA for Detection of Anti-Borrelia in Wildlife and Agricultural Animals.- A Step-by-Step Guide to Selecting an Optimal Cut-Off Value Based on the Receiver Operating Characteristic and Youden Index in Methods Designed to Diagnose Lyme Disease.- Adaptive Immune Response Investigation in Lyme borreliosis.- CYTOSPOT: Intracellular Staining and Detection of Cytokines by Flow Cytometer, An Alternative to LYMESPOT.- Use of Specific Borrelia Phages as a New Strategy for Improved Diagnostic Tests.- Identification of Unambiguous Borrelia Peptides in Human Urine Using Affinity Capture and Mass Spectrometry.- Preparation of Borrelia Infected Mammalian Cells for Helium Ion Microscopy.- Establishing a Zebrafish Model for Borrelia Burgdorferi Infection Using Immersion and Microinjection Methods.- Development of Organoids to Study Infectious Host Interactions.- Content Analysis: An Approach to Exploring the Depiction of Tick-Borne Diseases.- Applying BERT and ChatGPT for Sentiment Analysis of Lyme Disease in Scientific Literature.- Building a Binary Classification Machine Learning Model: A Guide to Predicting Participation in a Lyme Disease Program at a Medical Institute.- Clinical Algorithm and Diagnostic Tools for Lyme Disease.- Establishing a Pregnancy Lyme Disease Biobank.- Developing a Prospective Gestational Lyme Disease Study.
