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This second edition volume expands on the previous edition with discussions on the latest advancements in protein tyrosine phosphatases (PTP) research used to investigate these essential enzymes and new inhibitors. The new techniques covered in the chapters of this book include studying enzymes in vitro, in cells, and in animal models through proteomics, genomics, and structural biology. Furthermore, new advances in pharmacology and drug design have contributed to the developing novel therapeutics that target PTPs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Protein Tyrosine Phosphatases: Methods and Protocols, Second Edition is a valuable resource for both experienced and novel researchers in this field, and will lead to discoveries and accelerated progress in the field of PTP, signal transduction, and drug development.
List of contents
Induction of Translational Readthrough on Protein Tyrosine Phosphatases Targeted by Premature Termination Codon Mutations in Human Disease.- Understanding Pseudophosphatase Function through Biochemical Interactions.- CRISPR/Cas9-Mediated Modification of PTP Expression.- Osteoclast Methods in Protein Phosphatase Research.- OT-I TCR Transgenic Mice to Study the Role of PTPN22 in Anti-Cancer Immunity.- Protein-Tyrosine Phosphatases Studied in Zebrafish.- Examining Phosphatases through Immunofluorescent Microscopy.- Identification of Protein Tyrosine Phosphatase (PTP) Substrates.- Kinase-Catalyzed Biotinylation to Identify Phosphatase Substrates (K-BIPS).- Systems-Level Analysis of the Effects of RPTPs on Cellular Signaling Networks.- Detection of Protein Tyrosine Phosphatase Interacting Partners by Mass Spectrometry.- Detecting PTP Protein-Protein Interactions by Fluorescent Immunoprecipitation Analysis (FIPA).- Identifying Transmembrane Interactions in Receptor Protein Tyrosine Phosphatase Homodimerization and Heterodimerization.- Preparation of Oxidized and Reduced PTP4A1 for Structural and Functional Studies.- Measuring the Reversible Oxidation of Protein Tyrosine Phosphatases using a Modified Cysteinyl-Labeling Assay.- Identification and Optimization of Protein Tyrosine Phosphatase Inhibitors via Fragment Ligation.- Targeting Non-Conserved and Pathogenic Cysteines of Protein Tyrosine Phosphatases with Small Molecules.- In Vitro Phosphatase Assays for the Eya2 Tyrosine Phosphatase.- High Throughput Discovery and Characterization of Covalent Inhibitors for Protein Tyrosine Phosphatases.
