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This second edition volume expands on the previous edition with new and updated chapters that highlight the latest methods and approaches for the manipulation of RNA viruses. The chapters in this book explore the fundamental role in studying RNA viruses; identifying markers of host range, disease, and transmission; and aid readers with the further development of in silico computational biology tools and artificial intelligence algorithms that can help predict the emergence of certain pathogens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Reverse Genetics of RNA Viruses: Methods and Protocols, Second Edition is a valuable resource for researchers who are interested in learning more about this developing field.
List of contents
Reverse Genetics Systems for Filoviruses.- Reverse Genetics Systems for the de novo Rescue of Diverse Members of Paramyxoviridae.- Rescue of Recombinant Newcastle Disease Virus Expressing Heterologous Genes.- Use of Reverse Genetics for the Generation of Recombinant Influenza Viruses Carrying Nanoluciferase.- Reverse Genetics of Bat Influenza A Viruses.- Rescue of Infectious Salmon Anemia Virus (ISAV) from Cloned cDNA.- Reverse Genetics System for Rift Valley Fever Virus.- Plasmid-Based Lassa Virus Reverse Genetics.- Bacterial Artificial Chromosome Reverse Genetics Approaches for SARS-CoV-2.- Construction of Infectious Clones for Human Enteroviruses.- Reverse genetics of Hepatitis C Virus using an RNA Polymerase I-Mediated Transcription.- Reverse Genetics of Zika Virus using a Bacterial Artificial Chromosome.- A Stable Reverse Genetics System of Zika Virus Based on a Self-Splicing Group II Intron.- Reverse Genetics of Dengue Virus.- Recovery of Recombinant Rotaviruses by Reverse Genetics.
