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This detailed volume explores key concepts and experimental design related to Positron Emission Tomography (PET) imaging that have revolutionized our understanding of human biology. The first part focuses on recent advances in radiotracer probe development to enable the detection of materials, from large macromolecules to complicated drug-like structures. The next section describes how key physiological and pathophysiological processes can be interrogated and quantifiably measured with this imaging technique. Finally, chapters examine important technological developments in the field that are revolutionizing the way these innovative PET probes are utilized in the clinic. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Positron Emission Tomography: Methods and Protocols serves as an ideal guide for researchers looking to use imaging to revolutionize the way we diagnose and treat disease.
List of contents
11C-Fixation Techniques.- Enantioselective Synthesis of Carbon-11 Labeled Amino Acids and Peptides.- Silicon Fluoride Acceptors (SiFA) for 18F-Radiolabeling: From Bench to Bedside.- Tandem Iridium-Catalyzed C-H Borylation/Copper-Mediated Radiofluorination of Aromatic C-H Bonds with [18F]TBAF.- Preparation of [68Ga]GaCl3 Using a Cyclotron.- Transition and Post-Transition Radiometals for PET Imaging and Radiotherapy.- Rapid Radiolabeling for Peptide Radiotracers.- Methods for the Production of Radiolabeled Bioagents for ImmunoPET.- Radiolabeled Antibodies for Immune Checkpoint PET in Preclinical Research.- Radiolabeled Affibody Molecules for PET Imaging.- Small Animal Multisubject PET/CT Workflow.- The Basics of Visualizing, Analyzing, and Reporting Preclinical PET/CT Imaging Data.- Radiosynthesis of [18F]FPenM-C2Am: A PET Imaging Agent for Detecting Cell Death.- Imaging the Tumor Antioxidant Response with [18F]FSPG PET.- Characterization and Validation of Radiotracer Kinetics Using the Langendorff Isolated Perfused Heart.- Brain PET Imaging in Small Animals: Tracer Formulation, Data Acquisition, Image Reconstruction, and Data Analysis.- CRISPR/Cas for PET Reporter Gene Engineering.- Genetic Engineering of Therapeutic Cells with the Sodium Iodide Symporter (NIS) to Enable Non-Invasive In Vivo Therapy Tracking.- Single-Cell PET Imaging and Tracking.- PET System Technology: Theoretical Aspects and Experimental Methodology.- Practical Considerations for Total-Body PET Acquisition and Imaging.- Combined PET/MR: Where Anatomical Imaging Meets Cellular Function.- Quantitation of Oncologic Image Features for Radiomic Analyses in PET.- Multiplexing Autoradiography.- Kinetic Modeling Methods in Preclinical Positron Emission Tomography Imaging.
Report
"This book is a must-have in all PET research settings, where professionals from a multitude of areas will benefit from the detail of the description of the protocols, as well as the insight provided by the contextual materials included." (André Nunes, RAD Magazine, May, 2024)
