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This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This is the second of two volumes on endosome signaling and includes chapters on such topics as measurement of entry into the endosomal compartment by multi-parametric image analysis, assessment of peptide internalization and endosomal signaling, and VEGF-A in endosomal signaling.
List of contents
Section I. SIGNALING
1. Measurement of insulin proteolysis and assessment of insulin-mediated signaling (events) in (liver) endosomes
2. Endosomal sorting and implication for signaling
3. TGF-beta in endosomal signaling
4. Annexin A6 and endosomal signaling
5. PI-3-P in endosomal signaling
6.Endosomal signaling and cell migration
7. Measuring lipid-ginding effectors in endosomal signaling
8. Measuring role for endosomal signaling in tumorigenesis
9. Measurement of endosomal signaling in the immune system
10. Assessment of the TLR3 pathway in endosomal signaling
11. Labeling of platelet-derived growth factor to study its endocytosis and signaling
12. Endosomal signaling and oncogenesis
13. ROS - Containing endosomal compartments: implications for signaling
14. ERBIN in endosomal signaling
15. Lipid raft in endosomal signaling
16. VEGF-A in endosomal signaling
17. Assessment of peptide internalization and endosomal signaling
18. Quantitative proteomic analysis of compartmentalized signaling networks
19. Death signaling endosomes: sequential activation of Caspase-8, Caspase-9, acid sphingomyelinase and Cathepsin D in internalized TNF-receptosomes
20. Deubiquitinases and their emerging roles in cell signaling
21. NF-kB-dependent pathway in human keratinocytes
22. Endosomal signaling by the G protein coupled PARs
23. Investigating signaling consequences of GPCR trafficking in the endocytic pathway
24. MICAL-L1 in endosomal signaling
