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This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in biomineralization science, and includes sections on such topics as determining solution chemistry, structure and nucleation; probing structure and dynamics at surfaces; and interfaces mapping biomineral and morphology and ultrastructure.
List of contents
Determining solution chemistry, structure and nucleation
1. Development of accurate force fields for the simulation of biomineralisation
2. The integration of ion potentiometric measurements with chemical, structural and morphological analysis to follow mineralization reactions in solution.
3. Investigating the Early Stages of Mineral Precipitation by Potentiometric Titration and Analytical Ultracentrifugation
4. Replica Exchange Methods in Biomineral Simulations
5. SAXS in inorganic and bio-inspired research
6. In situ Solution Study of Calcium Phosphate Crystallization Kinetics
Probing structure and dynamics at surfaces and interfaces
7. Design, fabrication, and applications of in situ fluid cell TEM to mineralization reactions
8. X-ray Absorption Spectroscopy for the Structural Investigation of Self-Assembled-Monolayer-Directed Mineralization
9. Cryo-TEM Analysis of Collagen Fibrillar Stucture
Biomimetic crystallization techniques in vitro and in silico
10. Preparation of organothiol self-assembled monolayers for use in templated crystallization
11. Experimental techniques for the growth and characterization of silica biomorphs and silica gardens
12. Precipitation in liposomes as a model for intracellular biomineralization
13. Polymer Mediated Growth of Crystals and Mesocrystals
14. Phage display for the discovery of hydroxyapatite associated peptides
Protein structure, interactions and function
15. Quantitatively and Kinetically Identifying Binding Motifs of Amelogenin Proteins to Mineral Crystals through Biochemical and Spectroscopic Assays
16. Using the RosettaSurface Algorithm to Predict Protein Structure at Mineral Surfaces
17. Investigating Protein Function in Biomineralized Tissues using Molecular Biology Techniques
Mapping biomineral morphology and ultrastructure
18. Imaging the nanostructure of bone and dentin through small- and wide-angle scattering
19. Synchrotron X-ray Nanomechanical Imaging of Mineralized Fibre Composites
Mapping mineral chemistry
20. Application of Total X-ray Scattering Methods and Pair Distribution Function Analysis for Study of Structure of Biominerals
21. X-ray Microdiffraction of Biominerals
22. FT-IR and Raman studies of structure and bonding in mineral and organic-mineral composites
23. A mixed flow reactor method to synthesize amorphous calcium carbonate under controlled chemical conditions
