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This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This is the second of three volumes on hydrogen peroxide and cell signaling, and includes chapters on such topics as the cellular steady-state of H2O2, evaluating peroxiredoxin sensitivity towards inactivation by peroxide substrates, and peroxiredoxins as preferential targets in H2O2-induced signaling.
List of contents
1. The Cellular Steady-State of H2O2: Latency Concepts and Gradients
2. Evaluating Peroxiredoxin Sensitivity Towards Inactivation by Peroxide Substrates
3. Peroxiredoxins as Preferential Targets in H2O2-Induced Signaling
4. Selenium in the Redox Regulation of the Nrf2 and the Wnt Pathway
5. Selenoprotein W as Biomarker for the Efficacy of Selenium Compounds to Act as Source for Selenoprotein Biosynthesis
6. Peroxiredoxins and Sulfiredoxin at the Crossroads of the No and H2o2 Signaling Pathways
7. Glutathione and g-glutamylcysteine in Hydrogen Peroxide Detoxification
8. Peroxiredoxin-6 and NADPH Oxidase Activity
9. Study of the Signaling Function of Sulfiredoxin and Peroxiredoxin III in Isolated Adrenal Gland: Unsuitability of Clonal and Primary Adrenocortical Cells
10. The Use of HyPer to Examine Spatial and Temporal Changes in H2O2- in High Light-Exposed Plants
11. Analysis of Environmental Stress in Plants with the Aid of Marker Genes for H2O2 Responses
12. The Role of Plant Bax Inhibitor-1 in Suppressing H2O2-Induced Cell Death
13. Comparative Analysis of Cyanobacterial and Plant Peroxiredoxins and their Electron Donors: Peroxidase Activity and Susceptibility to Overoxidation
14. Using Hyper as a Molecular Probe to Visualize Hydrogen Peroxide in Living Plant cells: A Method with Virtually Unlimited Potential in Plant Biology
