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This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers cilia and includes chapters on such topics as methods for studying ciliary polarity in Xenopus, analysis of signaling pathways in mammalian spermatozoa, and biochemical and physiological analysis of axonemal dyneins.
List of contents
- Methods for studying ciliary polarity in Xenopus
- Analysis of signaling pathways in mammalian spermatozoa
- Analysis of tubulin modification in zebrafish cilia
- Analysis of microtubule end-binding proteins in cilia
- Small molecule screens using Chlamydomonas flagella
- Biochemical and Physiological analysis of axonemal dyneins
- Analysis of ciliary assembly and function in Planaria
- Reconstitution of flagellar sliding
- Comparative genomic analysis of ciliary genes
- Analysis of ciliogenesis in Tetrahymena
- Measuring the regulation of dynein activity during flagellar motility
- Centrosome isolation and proteomics
- Probing ciliogenesis using micropatterned substrates
- 3D structural analysis of flagella/cilia by electron cryo-tomography
- Visualizing IFT in mammalian cilia
- Ciliogenesis in multiciliated epithelial cells in culture
- Methods for analysis of PKD-containing urinary exosomes
- Analysis of Interactions between IFT Proteins
- Electron microscopy of Intraflagellar Transport
- Using the SNAP tag to measure ciliary trafficking
