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The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM.
List of contents
- Imaging Fluorescently Labelled Complexes by Means of Multidimensional Correlative Light and Transmission Electron Microscopy: Practical Considerations
- Visualising Live Dynamics and Ultrastructure of Intracellular Organelles with Pre-Embedding Correlative Light-Electron Microscopy
- Correlative Fluorescence and Transmission Electron Microscopy in Tissues
- Correlative Light and Electron Microscopy in Parasite Research
- Labeling of Ultrathin Resin Sections for Correlative Light and Electron Microscopy
- 3D HDO-CLEM: Cellular Compartment Analysis by Correlative Light-Electron Microscopy on Cryosection
- Correlative Light and Electron Microscopy of GFP
- Picking Faces Out of a Crowd: Genetic Labels for Identification of Proteins in Correlated Light and Electron Microscopy Imaging
- Correlated Light Microscopy and Electron Microscopy (CLEM): Search. and Find!
- Capturing Endocytic Segregation Events with HPF-CLEM
- Targeted Ultramicrotomy: a Valuable Tool for Correlated Light and Electron Microscopy of Small Model Organisms
- Correlative Light and Electron Microscopy of Intermediate Stages of Meiotic Spindle Assembly in the Early C. Elegans Embryo
- Precise, Correlated Fluorescence Microscopy and Electron Tomography of Lowicryl Sections Using Fluorescent Fiducial Markers
- Integrative Approaches for Cellular Cryo-Electron Tomography:Correlative Imaging and Focused Ion Beam Micromachining
- Visualizing Proteins in Electron Micrographs at Nanometer Resolution
- Atmospheric Scanning Electron Microscope for Correlative Microscopy
- Bridging Microscopes: 3D Correlative Light and Electron Microscopy of Complex Biological Structures
- Correlative Light and Volume EM: Using Focused Ion Beam Scanning Electron Microscopy to Image Transient Events in Model Organisms
