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This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. The volume covers nucleosomes, histones and chromatin and has chapters on dynamic mapping of histone-DNA interactions in nucleosomes by unzipping single molecules of DNA, digital DNase technology, and genome-wide analysis of chromatin transition.
List of contents
18. TBC
19. Quantitative affinity measurements for chromatin binding modules and their cognate marks-- pull-downs/ITC/FP/SPR
20. TBC
21. Modified Lysine Analogs for the study of chromatin modifying enzymes and nucleosome structure
22. Map the exact polydenylation site of mRNA transcripts
23. Analysis of the mechanism of chromatin remodeling by
24. TBC
25. Dynamic mapping of histone-DNA interactions in nucleosomes by unzipping single molecules of DNA
26. Mechanisms of ATP-dependent chromatin remodelling revealed by high-resolution optical tweezers
27. Enzymatic analysis of the Tet proteins by TLC and mass spectrometry
28. Investigating histone modifications using quantitative mass spectrometry
29. Single-molecule studies of chromatin remodeling
30. Genome-wide mapping of nucleosomes, chromatin modifications and chromatin-modifying enzymes
31. Digital DNase technology
32. Genome-wide Analysis of Chromatin Transitions; Integration of Static and Dynamic Approaches
33. Mapping the transcription machinery on the yeast genome
34. TBC
