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This volume covers the latest key aspects of therapeutic protein applications. Chapters in this book cover topics such as the discovery, production, and conjugation of protein-proteins with discussions on the direction of future development and advancements; ways to use these engineering proteins for therapeutic and vaccine applications; and the use of modified protein nanocarriers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Therapeutic Proteins: Methods and Protocols is a valuable resource for any researcher who are interested in learning more about the field of therapeutic proteins.
List of contents
Optimizing Cell-Free Protein Synthesis for Antimicrobial Protein Production.- Design and Construction of Antibody Fusion Proteins Incorporating Variable New Antigen Receptor (VNAR) Domains.- Selective and Site-Specific Incorporation of Non-Standard Amino Acids within Proteins for Therapeutic Applications.- An Approach for Antigen-Agnostic Identification of Virus-Like Particle-Displayed Epitopes that Engage Specific Antibody V Gene Regions.- Split-Protein Therapeutic Platforms: Identifying Binder Pairs.- Metal-Mediated Ligand Affinity Chemistry (MLAC).- Recombinant Elastin-Based Bioelastomers for Biomedical Applications.- Engineering Hepatitis B Virus (HBV) Protein Particles for Therapeutic Delivery.- Design and Purification of Tag/Catcher AP205-Based Capsid Virus-Like Particle Vaccines.- Protein Nanocarriers Capable of Encapsulating Both Hydrophobic and Hydrophilic Drugs.- Unnatrual Amino Acid Engineering for Intracellular Deliveryof Protein Therapeutics.- Formulation of Chitosan-Zein Nano-in-Microparticles for Oral DNA Delivery.- Engineering E2 Bionanoparticles for Targeted Delivery of Chemotherapeutics to Breast Cancer Cells.- Constructing Nucleic Acid Delivering Lipoproteoplexes from Coiled-Coil Supercharged Protein and Cationic Liposomes.- Cancer Cell Silicification and Surface Functionalization to Create Microbial Mimetic Cancer Vaccines.- Melt-Processing Virus-Like Particle-Based Vaccine Candidates into Biodegradable Polymer Implants.
