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Enzymes of Epigenetics: Part B, one of two new volumes in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field.
This volume covers research methods that are employed in the study of epigenetic regulation, including structural, biochemical, molecular, biological, cellular, computational, and systems approaches.
Topics include chromatin structure and histones, posttranslational histone modification enzymes and complexes, histone modification binders, DNA modifications and nucleic acid regulators, epigenetic technologies, and small molecule epigenetic regulators and biological connections.
List of contents
Epigenetic Technologies
1. Identification and Quantification of Histone PTMs using High-Resolution Mass Spectrometry
Kelly R. Karch, Simone Sidoli and Benjamin A. Garcia
2. Substrate Specificity Profiling of Histone-Modifying Enzymes by Peptide Microarray
Evan M. Cornett, Bradley M. Dickson, Robert M. Vaughan, Swathi Krishnan, Raymond C. Trievel, Brian D. Strahl, and Scott B. Rothbart
3. ArrayNinja: An Open Source Platform for Unified Planning and Analysis of Microarray Experiments
Bradley M. Dickson, Evan M. Cornett, Zachary Ramjan and Scott B. Rothbart
4. Chemical Biology Approaches for Characterization of Epigenetic Regulators
Dalia Barsyte-Lovejoy, Magdalena M Szewczyk, Panagiotis Prinos, Evelyne Lima-Fernandes, Suzanne Ackloo and Cheryl H. Arrowsmith
5. Mapping Lysine Acetyltransferase-Ligand Interactions by Activity-Based Capture
David C. Montgomery and Jordan L. Meier
6. Investigating Histone Acetylation Stoichiometry and Turnover Rate
Jing Fan, Josue Baeza and John M. Denu
7. Rapid Semisynthesis of Acetylated and Sumoylated Histone Analogs
Abhinav Dhall, Caroline E. Weller and Champak Chatterjee
8. An IF-FISH Approach for Co-Visualization of Gene Loci and Nuclear Architecture in Fission Yeast
Kyoung-Dong Kim, Osamu Iwasaki and Ken-ichi Noma
Small Molecule Epigenetic Regulators
9. Biology, Chemistry and Pharmacology of Sirtuins
Antonio Bedalov, Sarwat Chowdhury and Julian A. Simon
10. Synthesis and Assay of SIRT1 Activating Compounds
Han Dai , James L. Ellis , David A. Sinclair and Basil P. Hubbard
11. Synthesis and Assays of Inhibitors of Methyltransferases
Xiao-Chuan Cai, Kanishk Kapilashrami and Minkui Luo
Epigenetics and Biological Connections
12. Exploring the Dynamic Relationship between Cellular Metabolism and Chromatin Structure using SILAC-Mass Spec and ChIP-Sequencing
Philipp Mews and Shelley L. Berger
13. Current Proteomic Methods to Investigate the Dynamics of Histone Turnover in the Central Nervous System
Lorna A. Farrelly, Brian D. Dill, Henrik Molina, Marc R. Birtwistle and Ian Maze
14. ChIP-Sequencing to Map the Epigenome of Senescent Cells using Benzonase Endonuclease
Taranjit Singh Rai and Peter D. Adams
15. Exploiting Chromatin Biology to Understand Immunology
John L. Johnson and Golnaz Vahed
Report
Praise for the Series:
"Should be on the shelves of all libraries in the world as a whole collection." --Chemistry in Industry
"The work most often consulted in the lab." --Enzymologia
"The Methods in Enzymology series represents the gold-standard." --Neuroscience
