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Selection of the HPLC Method in Chemical Analysis serves as a practical guide to users of high-performance liquid chromatography and provides criteria for method selection, development, and validation.
High-performance liquid chromatography (HPLC) is the most common analytical technique currently practiced in chemistry. However, the process of finding the appropriate information for a particular analytical project requires significant effort and pre-existent knowledge in the field. Further, sorting through the wealth of published data and literature takes both time and effort away from the critical aspects of HPLC method selection.
For the first time, a systematic approach for sorting through the available information and reviewing critically the up-to-date progress in HPLC for selecting a specific analysis is available in a single book. Selection of the HPLC Method in Chemical Analysis is an inclusive go-to reference for HPLC method selection, development, and validation.
List of contents
1. Start of the Implementation of a New HPLC Method2. Short Overviews of Analytical Techniques Not Containing an Independent Separation Step3. Short Overviews of the Main Analytical Techniques Containing a Separation Step4. Basic Information Regarding the HPLC Techniques5. Properties of Analytes and Matrices Determining HPLC Selection6. General Aspects Regarding the HPLC Analytical Column7. RP-HPLC Analytical Columns8. Polar Analytical Columns9. Stationary Phases and Columns for Ion Exchange, Ion-Moderated, and Ligand Exchange Chromatography10. Stationary Phases and Columns for Chiral Chromatography11. Stationary Phases and Columns for Size Exclusion12. Stationary Phases and Columns for Immunoaffinity Type Separations13. Solvents, Buffers, and Additives Used in the Mobile Phase14. Gradient Elution15. The Practice of HPLC
