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Enzymes of Epigenetics, one of two new volumes in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field.
This volume covers research methods that are employed to study epigenetic regulation and includes structural, biochemical, molecular, biological, cellular, computational, and systems approaches.
Topics include chromatin structure and histones, posttranslational histone modification enzymes and complexes, histone modification binders, DNA modifications and nucleic acid regulators, epigenetic technologies and small molecule epigenetic regulators, and biological connections
List of contents
Chromatin Structure and Histones
1. In Vitro Chromatin Assembly - Strategies and Quality Control
Uma Muthurajan, Francesca Mattiroli, Serge Bergeron, Keda Zhou, Yajie Gu, Srinivas Chakravarthy, Pamela Dyer, Thomas Irving and Karolin Luger
2. Preparation, Crystallization and Structure Determination of Chromatin Enzyme/Nucleosome Complexes
Robert K. McGinty, Ravindra D. Makde and Song Tan
3. Preparation of Recombinant Centromeric Nucleosomes and Formation of Complexes with Non-Histone Centromere Proteins
Nikolina Sekulic and Ben E. Black
4. Functional Characterization of Histone Chaperones using SNAP-Tag-Based Imaging to Assess De Novo Histone Deposition
Camille Clément, Isabelle Vassias, Dominique Ray-Gallet and Geneviève Almouzni
5. Analysis of Nucleosome Sliding by ATP-Dependent Chromatin Remodelling Enzymes
Coral Y. Zhou and Geeta J. Narlikar
Posttranslational Histone Modification Enzymes and Complexes
6. In Vitro Activity Assays for Myst Histone Acetyltransferases and Adaptation for High-Throughput Inhibitor Screening
Cheryl E. McCullough and Ronen Marmorstein
7. Preparation and Biochemical Analysis of Classical Histone Deacetylases
Alejandro Villagra, Eva Sahakian and Edward Seto
8. Recombinant Preparation, Biochemical Analysis, and Structure Determination of Sirtuin Family Histone/Protein Deacylases
Benjamin Suenkel and Clemens Steegborn
9. Preparation, Biochemical Analysis and Structure Determination of SET Domain Histone Methyltransferases
Elisa Bergamin and Jean Francois Couture
10. Expression, Purification and Biochemical Analysis of the LSD1/KDM1A Histone Demethylase
Benoit Laurent and Yang Shi
11. LSD1 Histone Demethylase Assays and Inhibition
Dawn Hayward and Philip A. Cole
12. Purification, Biochemical Analysis, and Structure Determination of JmjC Lysine Demethylases
Swathi Krishnan and Raymond C. Trievel
13. Preparation and Analysis of Native Chromatin Modifying Complexes
Yannick Doyon and Jacques Côté
Histone Modification Binders (Readers)
14. Preparation, Biochemical Analysis and Structure Determination of the Bromodomain, an Acetyl-lysine Binding Domain
Chunyan Ren, Lei Zeng and Ming-Ming Zhou
15. Preparation, Biochemical Analysis and Structure Determination of Methyllysine Readers
Catherine A. Musselman and Tatiana G. Kutateladze
DNA Modifications and Nucleic Acid Regulators
16. Quantification of Oxidized 5-Methylcytosine Bases and TET Enzyme Activity
Monica Yun Liu, Jamie E. DeNizio and Rahul M. Kohli
17. Characterization of how DNA Modifications Affect DNA Binding by C2H2 Zinc Finger Proteins
Anamika Patel, Hideharu Hashimoto, Xing Zhang and Xiaodong Cheng
18. Crystallographic Studies of Telomerase
Hunter Hoffman and Emmanuel Skordalakes
19. Detection and Analysis of Long Non-Coding RNAs
Fan Lai, Ezra Blumenthal and Ramin Shiekhattar
20. Identifying Centromeric RNAs Involved in Histone Dynamics in Vivo
Delphine Quénet, David Sturgill and Yamini Dalal
Report
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"Should be on the shelves of all libraries in the world as a whole collection." --Chemistry in Industry
"The work most often consulted in the lab." --Enzymologia
"The Methods in Enzymology series represents the gold-standard." --Neuroscience
