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This volume summarizes key methods and advancements in the field of henipavirus molecular biology. Chapters guide readers through protocols focusing on emerging bat-borne paramyxoviruses as well as pathogenicity caused by Nipah and Hendra viruses. Protocols are also included to help develop diagnostic tools, antiviral therapeutics, and vaccine creation. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge, Nipah Virus: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.
List of contents
Overview of experimental vaccines and antiviral therapeutics for Henipavirus infection.-A Revised Diagnostic Quantitative RT-PCR for Detection of Nipah Virus Infection.- Recombinant Soluble Henipavirus Glycoprotein Preparation.- Cell-cell fusion assays to study henipavirus entry and evaluate therapeutics.- Recombinant Cedar virus: a henipavirus reverse genetics platform.- Utilizing Recombinant Reporter Henipaviruses to Conduct Antiviral Screening.- In vitro antiviral screening for henipaviruses at BSL4.- Isolation of primary porcine bronchial epithelial cells for Nipah virus infections.- Primary culture of the human olfactory neuroepithelium and utilization for henipavirus infection in vitro.- Mouse models of Henipavirus infection.- In vivo imaging of Nipah virus infection in small animal rodent models.- Nonhuman Primate Models for Nipah and Hendra Virus Countermeasure Evaluation.- Nipah virus aerosol challenge of three distinct particle sizes in nonhuman primates.- Generation and characterization of a humanized lung xenograft mouse model for studying henipavirus pathogenesis.- Ferret models for henipavirus infection.- Syrian golden hamster model for Nipah virus infection.- Anti-Nipah Virus Enzyme-Linked Immunosorbent Assays with Nonhuman Primate and Hamster Serum.- Assays for Detecting Henipavirus Antibodies.- Profiling host microRNA responses to henipavirus infection.- Host Transcriptome Analysis of Ferret Tissues Following Henipavirus Infection.
