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A compendium of optimized methods to measure type I interferon efficacy as an antiproliferative or an antiviral agent. These cutting-edge techniques range from the simple to the highly complex and serve to illuminate the signaling cascades and the activation of enzymatic pathways prompted by interferon. The protocols follow the successful Methods in Molecular Medicine series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
State-of-the-art and highly practical, Interferon Methods and Protocols offers researchers powerful tools not only to ascertain the functions of IFN-stimulatory gene products, but also to identify additional molecular pathways that will clarify our understanding of the many biological events influenced by IFNs.
List of contents
Biological and Clinical Basis for Molecular Studies of Interferons
Katie R. Pang, Jashin J. Wu, David B. Huang, Stephen K. Tyring, and Samuel Baron
Interferon Research: A Brief History
Myriam S. Kunzi and Paula M. Pitha
Virus Infection and the Interferon Response: A Global View Through Functional Genomics
Marcus J. Korth, John C. Kash, Jeffrey C. Furlong, and Michael G. Katze
Genomic DNA Affinity Chromatography: A Technique to Isolate Interferon-Inducible DNA Binding Factors
Jyothi Kumaran and Eleanor N. Fish
Protein Engineering of Interferon Alphas
Renqiu Hu, Ke-jian Lei, Joseph Bekisz, and Kathryn C. Zoon
Assays for the Interferon-Induced Enzyme 2',5' Oligoadenylate Synthetases
Saumendra N. Sarkar, Mitali Pandey, and Ganes C. Sen
A Convenient and Sensitive Fluorescence Resonance Energy Transfer Assay for RNase L and 2',5' Oligoadenylates
Chandar S. Thakur, Zan Xu, Zhengfu Wang, Zachary Novince, and Robert H. Silverman
Regulation of Murine Interferon Regulatory Factor Gene Expression in the Central Nervous System Determined by Multiprobe RNase Protection Assay
Shalina S. Ousman and Iain L. Campbell
Mitogen-Activated Protein Kinase Pathways in Interferon Signaling
Antonella Sassano, Amit Verma, and Leonidas C. Platanias
Development of an Interferon-Based Cancer Vaccine Protocol: Application to Several Types of Murine Cancers
W. Robert Fleischmann, Jr. and Tzu G. Wu
Type I Interferons as Regulators of the Differentiation/Activation of Human Dendritic Cells: Methods for the Evaluation of IFN-Induced Effects
Stefano M. Santini, Caterina Lapenta, and Filippo Belardelli
Flow Cytometric Techniques for Studying Plasmacytoid Dendritic Cells in Mixed Populations
Stacey L. Olshalsky and Patricia Fitzgerald-Bocarsly
Analysis of Anti-Interferon Properties of the Herpes Simplex Virus Type I ICP0 Protein
Karen Mossman
Interferon Subtype Gene Therapy for Regulating Cytomegalovirus Disease
Cassandra M. James, Emmalene J. Bartlett, Josephine P. Mansfield, and Vanessa S. Cull
Transfection of Müller Cells With Type I Interferon Transgenes: Resistance to HSV-1 Infection
Benitta John-Philip and Daniel J. J. Carr
Index
Summary
A compendium of optimized methods to measure type I interferon efficacy as an antiproliferative or an antiviral agent. These cutting-edge techniques range from the simple to the highly complex and serve to illuminate the signaling cascades and the activation of enzymatic pathways prompted by interferon. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
State-of-the-art and highly practical, Interferon Methods and Protocols offers researchers powerful tools not only to ascertain the functions of IFN-stimulatory gene products, but also to identify additional molecular pathways that will clarify our understanding of the many biological events influenced by IFNs.
