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This volume provides methods on the role of TLRs in immune responses and on the various approaches targeting different TLRs in cancer, autoimmunity and in vaccine development. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Toll-Like Receptors: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.
List of contents
Modelling of transmembrane domain and full length TLRs in membrane models.- Development of Optimal Virtual Screening Strategies to Identify Novel Toll-like Receptor Ligands using the DockBox Suite.- Use of Fluorescent Chemical Probes in the Study of Toll-like Receptors (TLRs) Trafficking.- Use of Fluorescent Chemical Probes in the Study of Toll-like Receptors (TLRs) Trafficking.- Endotoxin-tolerance mimicking to study TLR in promotion of tolerogenic DCs and Tr1 cells.- Flow cytometry analysis of IL-1 receptors and Toll like receptors on platelets and platelet-derived extracellular vesicles.- Methods to study TLRs in transplantation.- Determining endosomal Toll-like Receptors gene expression in NK cells after stimulation with specific agonists.- In vitro study of TLR4-NLRP3-inflammasome activation pathway in innate immune response.- Toll-like receptor polymorphisms and the risk of cancer: meta-analysis study.- Unrevealing the role of TLRs in the pathogenesis of autoimmune disease by using mouse model of diabetes.- In vitro and ex vivo methodologies for T cell trafficking through Blood Brain Barrier after TLR activation.- Delineating the role of Toll-like receptors in inflammatory bowel disease.- Analysis of differential TLR activation in a mouse model of multiple sclerosis.- Study of agonists of TLRs as vaccine adjuvants.- Activation of TLRs by opportunistic fungi in lung organoids.
