Read more
This collection provides methods and techniques employed when culturing, manipulating, quantifying, and functionally assessing retinal ganglion cells (RGCs), vital for better understanding various ocular diseases and for developing novel therapies. The book features exhaustive and detailed protocols for the study of RGCs at both the in vitro and in vivo level, including the culture of rodent and human cells, and immunohistochemical, morphological, and functional assessments of various in vivo models of RGC death. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Retinal Ganglion Cells: Methods and Protocols serves as an ideal guide to these important cellularfeatures of the central nervous system and the many traumatic and degenerative blinding diseases that affect them.
List of contents
Isolation and Culture of Primary Retinal Ganglion Cells from Rodent Retina.- Purification of Retinal Ganglion Cells from Differentiation through Adult Via Immunopanning and Low-Pressure Flow Cytometry.- Preparation of Retinal Explant Cultures.- Gene Gun DiOlistic Labeling of Retinal Ganglion Cells.- Differentiation of Human Embryonic/Induced-Pluripotent Stem Cells to Retinal Ganglion Cells.- Laser-Induced Ocular Hypertension in a Mouse Model of Glaucoma.- Silicone Oil-Induced Ocular Hypertension Glaucoma Model (SOHU) in Rodent and Non-Human Primate.- Microbead-Induced Ocular Hypertension in a Rodent Model of Glaucoma.- Viral Vector-Induced Ocular Hypertension in Mice.- Optic Nerve Crush Injury in Rodents to Study Retinal Ganglion Cell Neuroprotection and Regeneration.- Optical Coherence Tomography: Imaging Visual System Structures in Mice.- Confocal Scanning Laser Ophthalmoscopy to Image Retinal Ganglion Cells in Real-Time.- In Vivo Detection of Retinal Ganglion Cell Stress in Rodentswith DARC.- Measuring the Full-Field Electroretinogram in Rodents.- Evaluation of Retinal Ganglion Cell Via Visual Evoked Potential.- High-Throughput Binocular Pattern Electroretinograms in the Mouse.- Intravitreal Injection of AAV for the Transduction of Mouse Retinal Ganglion Cells.- Methods to Identify Rat and Mouse Retinal Ganglion Cells in Retinal Whole Mounts.
