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This protocol book presents reproducible and step-by-step procedures for the peptide synthesis, their characterization and applications. The volume includes an introductory section on in silico modelling of new peptide molecules, Molecular Dynamics Simulations, Docking, Electrostatic fingerprinting of peptides, and other modelling tools for peptide designing and optimization. Further, it covers protocols for the solid phase peptide synthesis, chromatographic and mass spectrometric characterization of peptides. Importantly, it covers methods for biophysical characterizations of peptides for their potential applications as drug delivery vehicles, peptide nano-assembly, bionanocatalysis, protein aggregation diseases, and peptide-based anti-bacterial.
List of contents
Dedication.- Preface.- Acknowledgement.- Chapter 1 De novo Peptide Design Tools.-Chapter 2 Molecular Docking.- Chapter 3 Molecular Dynamics Simulations.- Chapter 4 Peptide Synthesis.- Chapter 5 Mass Spectrometry .- Chapter 6 Circular Dichroism (CD) Spectroscopy.- Chapter 7 Fourier Transform Infrared (FTIR) Spectroscopy.- Chapter 8 Cell Culture for Cell Penetrating Peptides.- Chapter 9 Comparative Uptake of Peptides by Flow Cytometry.- Chapter 10 Cellular uptake of peptides by confocal microscopy.- Chapter 11 Penetration in 3D Tumorospheres.- Chapter 12 TMRM Cytotoxicity Assay.- Chapter 13 Cell Death by Apoptosis.- Chapter 14 Hemolytic activity.- Chapter 15 Antimicrobial Assay.- Chapter 16 Antimicrobial assay with Peptide Based Hydrogels.- Chapter 17 Rheology of Peptide based Hydrogels.- Chapter 18 Estimation of Aggregation Kinetics.- Chapter 19 Assessment of Amyloid morphology using Electron Microscopy.- Chapter 20 Catalytic Efficiency of the Designed Peptides.
