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This detailed volume expands upon the previous edition with key methods currently used in lymphoma research, partly specific for lymphoma research but often adaptable to the study of other cancers. New chapters explore the latest approaches for single cell B cell and T cell receptor sequencing, multiplexed immunophenotyping of lymphoma tissue samples, genetic manipulation and extended culture of human germinal center B cells, genetic mouse models of lymphomas, establishment of patient-derived xenograft models of lymphomas, and more. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-day, Lymphoma: Methods and Protocols, Third Edition serves as a valuable resource for hematologists, hematopathologists, and scientists interested in a variety of topics in cancer research, human genetics, and immunology.
List of contents
B Cell Differentiation and the Origin and Pathogenesis of Human B Cell Lymphomas.- Flow Cytometry for Non-Hodgkin and Hodgkin Lymphomas.- Laser-Based Microdissection of Single Cells from Tissue Sections and PCR Analysis of Rearranged Immunoglobulin Genes from Isolated Normal and Malignant Human B Cells.- PCR GeneScan Analysis of Rearranged Immunoglobulin or T-Cell Receptor Genes for Clonality Diagnostics in Suspect Lymphoproliferations.- Expression Cloning of Antibodies from Single Human B Cells.- Stereotyped B Cell Receptor Immunoglobulins in B Cell Lymphomas.- Flow Cytometric MRD Detection in Selected Mature B-Cell Malignancies.- MRD Detection in B-Cell Non-Hodgkin Lymphomas Using Ig Gene Rearrangements and Chromosomal Translocations as Targets for Real-Time Quantitative PCR and ddPCR.- FISH and FICTION in Lymphoma Research.- Genotype from Phenotype: Using CRISPR Screens to Dissect Lymphoma Biology.- Lymphoma and Leukemia Cell Vulnerabilities and Resistance Identified by Compound Library Screens.- Use of Proximity Ligation Assay to Study Lymphoid Malignancies.- Phosphoproteomic Analysis of Signaling Pathways in Lymphomas.- Single Cell VDJ Sequencing of Normal and Malignant B and T Cells.- Single-Cell Transcriptomic Analysis of Normal and Malignant B Cells.- Multiplexed Immunophenotyping of Lymphoma Tissue Samples.- Genetic Manipulation and Extended Culture of Human Germinal Center B Cells to Model Lymphomagenesis.- Genetic Mouse Models of Lymphomas.- Establishing Patient-Derived Xenograft (PDX) Models of Lymphomas.- Detection and Characterization of Clonal Hematopoiesis.- Detection of Circulating Tumor DNA in Lymphoma Patients.
