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Isothermal Titration Calorimetry in Enzymology: Techniques and Applications provides a thorough, practical overview of ITC as a productive and powerful tool for quantifying enzyme catalysis and interactions with substrates, products, cofactors, and inhibitors. Here, global experts in the methodology discuss the full capabilities of ITC and how it may be applied in research, medicine, and industry and offer a basis to continue leveraging ITC as its uses expand and evolve. This book bridges the gap between research and application by giving extensive technical details on cutting edge ITC techniques across a wide swath of both ITC kinetics measurements and ITC thermodynamic measurements, alongside case studies illustrating the depth of knowledge that can be obtained. Applications discussed include measuring binding kinetics by ITC, analyzing ITC kinetic data, in vivo enzyme kinetics by ITC, analyzing enzyme kinetics in crowded solutions, studying catalysis of biological substrates, interpreting ITC binding curves, ITC in drug discovery, ITC in the development of cancer therapeutics, ITC analysis of aminoglycoside resistance enzymes, studying enzyme interactions with toxic metals, using ITC to study ubiquitination, and ITC studies of glycoside hydrolases, among others.
List of contents
PART 1: ITC kinetics measurements1. Enzyme kinetics by ITC
2. Measuring binding kinetics by ITC
3. Analysing ITC kinetic data with AFFinimeter software
4. In vivo enzyme kinetics by ITC
5. Characterizing enzyme kinetics by 2D-ITC
6. Measuring enzyme stability using ITC
7. Kinetics of immobilized enzymes
8. Enzyme kinetics in crowded solutions
9. Kinetics of nanozymes
10. Using ITC to characterize enzyme allostery
11. Signal amplification in ITC kinetics experiments
12. ITC kinetic studies of 6-phytase
13. Catalysis of biological substrates of nuclear metallohydrolases
14. Alpha-amylase activity and inhibition
15. Enzyme stability and activity of psycrophillic enzymes
16. Non-MM kinetics of peroxidases and application to industrial wastes
17. Kinetics of diphosphohydrolases
PART 2: ITC thermodynamic measurements18. Interpretation of ITC binding curves
19. Analysis of ITC binding data using SEDPHAT
20. ITC in drug discovery
21. ITC in the development of cancer therapeutics
22. Mechanisms of aminoglycoside resistance enzymes
23. Using ITC to characterize the interactions of natural products with biomolecules
24. ITC studies of metalloenzymes
25. Enzyme interactions with toxic metals
26. Thermodynamics of molecular machines
27. Thermodynamics of protein chaperones
28. Characterizing protein interactions in apoptosis by ITC
29. Using ITC to study ubiquitination
30. Thermodynamics of transition state analogs
31. Thermodynamics of recombinant enzymes in biotechnology
32. Ion Binding to Transport Proteins using ITC
33. Urease from Helicobacter pylori, lessons from ITC
34. Substrate recognition by Chitinases
35. ITC studies of glycoside hydrolases
About the author
Associate Professor Anthony Mittermaier works in the Department of Chemistry, McGill University, Montreal, Canada.Justin Di Trani is an Assistant Professor at the University of Alberta, Department of Biochemistry, Alberta, Canada
