Read more
This detailed volume explores the use of single-cell assays in research for drug discovery, microfluidics, and more. The book delves into methodologies involving a variety of cell types and diseases, small molecules and biologics, as well as studies of the genome and transciptome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Single-Cell Assays: Microfluidics, Genomics, and Drug Discovery serves to enable researchers to obtain a rapid overview in state-of-the-art microfluidic single-cell assays and an impression of what possibilities these assays offer to drug discovery.
List of contents
Detection of Single Non-Small Cell Lung Cancer Cell Multi-Drug Resistance with Single Cell Bioanalyzer.- Cytosolic Calcium Measurement Utilizing a Single Cell Biochip to Study the Effect of Curcumin and Resveratrol on a Single Glioma Cell.- Dielectrophoresis-Assisted Self-Digitization Chip for High-Efficiency Single-Cell Analysis.- Optical pH Monitoring in Microdroplet Platforms for Live Cell Experiments Using Colloidal Surfactants.- PicoShells: Hollow Hydrogel Microparticles for High-Throughput Screening of Clonal Libraries.- Single Cell Micropatterning by Non-Fouling Hydrogels.- Microfluidics-Enabled Isolation and Single-Cell Analysis of Circulating Tumor Cells.- Discrimination of Multidrug Resistance in Cancer Cells Achieved Using Single-Cell Analysis.- Microfluidic Approach for Modelling Coupled Circadian Clock.- A High-Throughput Single-Cell Assay on a Valve-Based Microfluidic Platform Applied to Protein Quantification, Immune Response Monitoring, and Drug Discovery.- Single-Cell Exogenous Gene Transfection Analysis Chip.- Double Emulsion Flow Cytometry for Rapid Single Genome Detection.- Single-Cell Analysis of circRNA Using ddPCR.- A SINC-Seq Protocol for the Analysis of Subcellular Gene Expression in Single Cells.- Profiling Single-Cell Genome and Transcriptome by scONE-Seq.- Single Extracellular Vesicle Analysis Using Droplet Microfluidics.
