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This detailed book explores the interactions between different types of cells and immune cells, focusing on how their functionality is affected by immunosenescence. Beginning with traditional approaches, such as quantifying TRECs and measuring telomere lengths, the book continues with chapters on characterization of immune cell populations in aging, immune cell signaling, transcription factors, gene expression profiles, and the production of soluble factors such as cytokines, as well as computational approaches and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Immunosenescence: Methods and Protocols serves as an ideal guide for researchers working in this vital area of immunology and cell biology.
List of contents
Quantification of T Cell Receptor Excision Circles (TRECs).- Telomere Length Measurement in Human Tissue Sections by Quantitative Fluorescence In Situ Hybridization (Q-FISH).- Phenotypic Characterization of B Lymphocytes Subpopulations in Human Peripheral Blood: A Cost Effective 7-Color 1-Tube Protocol.- Method to Assess Immunosenescent CD8+ T Cells in Respiratory Viral Infections.- Gammad T Cell Immunophenotype for the Study of Human Aging.- Investigation of Signaling in Primary Populations of Human Senescent T Cells.- Transcription Factors Analysis to Investigate Immunosenescence in Rheumatoid Arthritis Patients.- Evaluation of Gene Expression Profiling by QuantiGeneTM 2.0 RNA Assay.- Application of Multiplex Immunoassay in Aging Research: A Methodological Approach.- Analysis of Cytokine-Inducible SH2-Containing Protein (CISH) in the Study of Immunosenescence.- Evaluating the Efficacy of Immune Checkpoint Inhibitors in Elderly Patients: A Systematic Review and Meta-Analysis.- Computational Analysis of T-Cell Receptor Repertoire Workflow: From T-Cell Isolation to Bioinformatics Analysis.- In Vitro Interaction Between Yeast Extracellular Vesicles and Human Monocyte-Derived Dendritic Cells.- Recording of Age-Related Changes on Murine and Human Brain Slices.- Immunofluorescence Staining of Murine Spinal Cord Sections to Evaluate Microglial Activation and Astrogliosis.- Characterization of Age-Dependent Changes in Skeletal Muscle Repair and Regeneration Using a Mouse Model of Acute Muscle Injury.- Inducing Cellular Senescence in Mouse Embryonic Fibroblasts (MEFs).- Polyphenol Treatment of Peripheral Blood Mononuclear Cells from Individuals of Different Ages.- Antioxidant Features of Humic Products by ABTS Assay.
