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This second edition volume presents new and updated protocols on comprehensive compendium of clinical metabolomics protocols covering LC-MS, GC-MS, CE-MS, and NMR-based clinical metabolomics as well as bioinformatics and study design considerations. Chapters explore the core of several promising initiatives evolving around personalized health care and precision medicine. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Clinical Metabolomics: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.
List of contents
(Pre)Clinical Metabolomics Analysis.- Global Metabolomics using LC-MS for Clinical Applications.- Modular, scalable and customizable LC-HRMS for exposomics.- Distinguishing artifactual fatty acid dimers from FAHFAs in untargeted LC-MS pipelines.- Advances in Chiral Metabolomic Profiling and Biomarker Discovery.- Stable Isotope Tracing Experiments using LC-MS.- Quantification of Total Ketone Bodies in biological matrices using Stable Isotope-labeled Internal Standards and RP-UPLC-MS/MS.- LC-MS/MS quantification of endocannabinoids in tissues.- Prostaglandin Metabolites Analysis in Urine by LC-MS/MS.- A method for analysis of oxidized phospholipids from biological samples using mass spectrometry.- Non-enzymatic oxidized polyunsaturated fatty acid products in human plasma and urine samples by LC-QTOF-MS/MS.- Reversed-Phase Ultrahigh-Performance Liquid Chromatography - Mass Spectrometry Method for High-Throughput Lipidomic Quantitation.- High-Throughput RPLC-MS/MS Quantification of Short- and Medium-Chain Fatty Acids.- Long chain base profiling with multiple reaction monitoring mass spectrometry.- Sphingolipid analysis in clinical research.- Recommendations for accurate lipid annotation and semi-absolute quantification from LC-MS/MS datasets.- Rapid analysis of Sterols in Blood Derived Samples.- Ultrahigh-Performance Supercritical Fluid Chromatography - Mass Spectrometry in the Clinical Lipidomic Analysis.- Quantification of octadecanoids in human plasma using chiral supercritical fluid chromatography-tandem mass spectrometry.- Rapid Lipid Mediator Profiling by Convergence Chromatography-MS/MS.- Analysis of the Mammalian Lipidome by DMS Shotgun Lipidomics.- Differential mobility spectrometry-based cardiolipin analysis.- CE-MS-based clinical metabolomics of human plasma.- Quantitative blood serum IVDr NMR spectroscopy in clinical metabolomics of cancer, neurodegeneration and internal medicine.- Lipoprotein (sub)fraction analysis on the Bruker B.I. LISA platform.- NMR-based stable isotope tracing of cancer metabolism.- NMR-based analysis of cellular central energy metabolism and exchange ratesIn Situ Isotope.- Tracing at Single-Cell Resolution Using Mass Spectrometry Imaging.- Extracting knowledge from MS clinical metabolomic data: Processing and analysis strategies.- Application of a computational metabolomics workflow for the diagnosis of inborn errors of metabolism in a laboratory setting.
