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This volume explores the latest techniques used in the field of mammalian germ cell biology to study key experimental approaches and perspectives for critical analysis of spermatogonial subsets including the rare spermatogonial stem cells (SSCs) pool. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding common pitfalls.
Cutting-edge and comprehensive, Spermatogonial Stem Cells: Methods and Protocols is a valuable resource for both new and experiences investigators looking to expand their knowledge in this developing field.
List of contents
Introduction: The Why's and How's for Studying Spermatogenesis and Spermatogonial Stem Cells.- Optimizing Testicular Fixation-Embedding Procedures for an Improved Morphofunctional Evaluation of Mammalian Spermatogonia.- Single-Molecule Fluorescence In Situ Hybridization for Spatial Detection of mRNAs in Sections of Mammalian Testes.- Bulk and Single-Cell RNA-Seq Analyses for Studies of Spermatogonia.- Profiling the Epigenetic Landscape of the Spermatogonial Stem Cell - Part 1: Epigenomics Assays.- Profiling the Epigenetic Landscape of the Spermatogonial Stem Cell - Part 2: Computational Analysis of Epigenomics Data.- Primary Cultures of Spermatogonia and Testis Cells.- Reconstitution of Human Prospermatogonial Development from Human Induced Pluripotent Stem Cells.- RNA Interference as a Method of Gene Knockdown in Cultured Spermatogonia.- Isolation of Undifferentiated Spermatogonia from Adult and Developing Mouse Testes.- Spermatogonial Transplantation.- Viral Transduction of Mammalian Spermatogonial Stem Cells.- Pharmacological Treatment of Neonatal and Juvenile Mice to Study Spermatogenesis.- Models and Methods for Evaluating Regeneration of Spermatogenesis After Cytotoxic Treatments.- Defining Gene Function in Spermatogonial Stem Cells through Conditional Knockout Approaches.- Lineage Tracing of Spermatogonial Stem Cells within the Male Germline.- Perspectives: Approaches for Studying Livestock Spermatogonia.- Perspectives: Methods for Evaluating Primate Spermatogonial Stem Cells.
