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This volume explores the latest collection of cell models that are used in preclinical cancer research, and covers both two-dimensional and three-dimensional culturing techniques. The chapters in this book are divided into two parts. Part One discusses two-dimensional cancer cell culture, cell models at the Air-Liquid Interface, and the latest advancements in three-dimensional complex spheroid models and dedicated disease animal models. Part Two contains technical chapters that illustrate step-by-step methodologies for specific cancer cell culture methods. The methods discussed range from the generation of isogenic cancer cell lines, the use of serum-free growth conditions, and three-dimensional cell cultures and their specific assays for the efficacy assessment of new anticancer therapies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Cancer Cell Culture: Methods and Protocols is a valuable tool to help researchers involved in this important field to further improve or advance their models for cancer research.
List of contents
Cancer Cell Culture: The Basics and Two-Dimensional Cultures.- Cell Cultures at the Air-Liquid Interface and Their Application in Cancer Research.- Three-Dimensional Spheroids for Cancer Research.- Disease Animal Models for Cancer Research.- Generation of Radio-Resistant Prostate Cancer Cells.- Generation and Characterization of an Isogenic Cell Line Model of Radioresistant Oesophageal Adenocarcinoma.- Developing an In Vitro Isogenic Model of Chemotherapy-Resistant Lung Cancer.- Culturing Human Lung Adenocarcinoma Cells in a Serum-Free Environment.- A Method for Culturing 3D Tumoroids of Lung Adenocarcinoma Cells at the Air-Liquid Interface.- Isolation and Cryopreservation of Mononuclear Cells from Peripheral Blood and Bone Marrow of Blood Cancer Patients.- Serum-Free Production of Human Stem Cell-Derived Liver Spheres for Cancer Metastasis Research.- A Self-Assembly Method for Creating Vascularized Tumor Explants Using Biomaterials for 3D Culture.- A Step-by-Step Methodological Guide for Developing Zonal Multicellular Scaffold-Based Pancreatic Cancer Models.- Measuring Immune Cell Movement Towards the Soluble Microenvironment of Human Tissues Using a Boyden Chamber-Based Migration Assay.- A Method for the In Vitro Cytotoxicity Assessment of Anti-Cancer Compounds and Materials Using High Content Screening Analysis.- Testing the Effects of Magnetic Hyperthermia in In Vitro 2D Tumor Cultures.- Cell Viability Assay with 3D Prostate Tumor Spheroids.- Analysis of Cancer Cell Line Secretomes: A Complementary Source of Disease-Specific Protein Biomarkers.
