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The latest, most efficient methods for the synthesis of peptides and proteins. This reference evaluates the most important synthesis methods available today, and also considers methods that show promise for future applications. This text describes the state of the art in efficient synthetic methods for the synthesis of both natural and artificial large peptide and protein molecules. Subjects include an introduction to basic topics, linear solid-phase synthesis of peptides, peptide synthesis in solution, convergent solid-phase synthesis, methods for the synthesis of branched peptides, formation of disulfide bridges, and more. The book emphasizes strategies and tactics that must be considered for the successful synthesis of peptides. TOC:Introduction. Amino Acids and Peptide and Protein Structure. Amino Acid Activation and Coupling. Protection Schemes for Peptide Synthesis. The Synthesis of Peptides. Solid- Phase Peptide Synthesis. The Solid Support. Protection Schemes. Attachment of the First Amino Acid to the Solid Support. Chain Elongation. Cleavage of the Peptide from the Resin. Examples of Protein Synthesis by Linear SPPS. Peptide Synthesis in Solution. Strategic Considerations. Protection Schemes. Chain Elongation. Final Removal of Protecting Groups and more.
List of contents
IntroductionAmino Acids and Peptide and Protein StructureAmino Acid Activation and CouplingProtection Schemes for Peptide SynthesisThe Synthesis of PeptidesSolid-Phase Peptide SynthesisThe Solid SupportProtection SchemesAttachment of the First Amino Acid to the Solid SupportChain ElongationCleavage of the Peptide from the ResinExamples of Protein Synthesis by Linear SPPSPeptide Synthesis in SolutionStrategic ConsiderationsProtection SchemesChain ElongationFinal Removal of Protecting GroupsSpecial Approaches to the Synthesis of Peptides in SolutionExamples of Peptide Synthesis in SolutionConvergent Approaches to the Synthesis of Large Peptides and ProteinsConvergent Solid-Phase Peptide SynthesisThe Coupling of Protected Peptide Segments in SolutionCoupling of Minimally Protected Peptides in Aqueous SolutionChemical Ligation of Peptide SegmentsFormation of Disulfide BridgesPeptides with Disulfide BridgesProtection of Cysteine in Peptide SynthesisChemical Methods for the Formation of Disulfide BridgesRegioselective Disulfide Bridge FormationPeptide LibrariesMultiple Peptide Synthesis in ParallelPeptide Library TechniquesExamples of Peptide Library SynthesisiEach chapter contains References
About the author
Paul Lloyd-Williams (Universitat de Barcelona, Barcelona, Spain), Fernando Albericio (Parc Cientific de Barcelona, Spain) and Ernest Giralt (Biomedical Research Institute, Barcelona, Spain)
Summary
Organic chemists working on the synthesis of natural products have long found a special challenge in the preparation of peptides and proteins
