Capillary Electrophoresis of Proteins

"Provides practical information on the application of capillary electrophoresis (CE) to protein analysis, with an emphasis on developing and optimizing CE techniques in the laboratory. Includes separation methods bases on mass, charge, isoelectric point, molecular sieving, and affinity interactions."

List of contents

Principles and practice of capillary electrophoresis: basic concepts; brand broadening in capillary electrophoresis; capillary electrophoresis intrumentation; preparative capillary electrophoresis; capillary electrophoresis separation modes. Detection of proteins: absorbance detection; fluorescence detection; mass spectrometry. Strategies for reducing protein-wall interactions: operations at pH extremes; use of buffer additives; capillary coatings and other surface modifications. Capillary zone electrophoresis: developing a CZE method; sample preconcentration techniques; applications. Capillary isoelectric focusing: sample preparation and injection; focusing; two-step CIEF; single-step CIEF; capillary selection; detection; optimizing CIEF analysis; capillary IEF as a micro-preparative technique; applications; future developments. Sieving separation: analysis of native proteins; analysis of SDS-protein complexes. Concluding remarks.

About the author

Tim Wehr, Roberto Rodriguez-Diaz, Mingde Zhu

Summary

"Provides practical information on the application of capillary electrophoresis (CE) to protein analysis, with an emphasis on developing and optimizing CE techniques in the laboratory. Includes separation methods bases on mass, charge, isoelectric point, molecular sieving, and affinity interactions."

Product details

• Assisted by: Roberto Rodriguez-Diaz (Editor), Rodriguez-Diaz Roberto (Editor), Tim Wehr (Editor), Wehr Tim (Editor), Mingde Zhu (Editor), Zhu Mingde (Editor)
• Publisher: Taylor and Francis
• Languages: English
• Product format: Hardback
• Released: 20.10.1998
• EAN: 9780824702052
• ISBN: 978-0-8247-0205-2
• No. of pages: 300
• Weight: 720 g
• Series: Chromatographic Science Series
• Subjects: Natural sciences, medicine, IT, technology > Technology > Chemical engineering; SCIENCE / Life Sciences / Biology, SCIENCE / Life Sciences / Biochemistry, SCIENCE / Chemistry / Organic, SCIENCE / Chemistry / Analytic, biochemistry, proteins, Polymer chemistry, Analytical Chemistry, Chemical biology