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This volume provides comprehensive dry and wet experiments, methods, and applications on nanopore sequencing. Chapters guide readers through bioinformatic procedures, genome sequencing, analysis of repetitive regions, structural variations, rapid and on-site microbial identification, epidemiology, and transcriptome analysis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge, Nanopore Sequencing: Methods and Protocols aims to be comprehensive guide for researchers.
List of contents
The current state of nanopore sequencing.- Hybrid genome assembly of short and long reads in Galaxy.- Microbial genome sequencing and assembly using nanopore sequencers.- De novo genome assembly of Japanese Black cattle as model of an economically relevant animal.- How to sequence and assemble plant genomes.- Detection of DNA modification using nanopore sequencers.- Ultra-low input genome library preparation for nanopore sequencing with droplet-MDA.- The method of eliminating the Wolbachia endosymbiont genomes from insect samples prior to long-reads.- A nanopore sequencing course for graduate school curriculum.- A guide to genome sequencing containing ultra-long repetitive regions.- Analysis of tandem repeat expansions using long DNA reads.- Finding rearrangements in nanopore DNA reads with last and dnarrange..- Long read whole-genome sequencing using a nanopore sequencer and detection of structural variants in cancer genomes.- Full-length 16S rRNA gene analysis using long-read nanopore sequencing for rapid identification of bacteria from clinical specimens.- Nanopore sequencing data analysis of 16S rRNA genes using Genome Sync-GSTK system.- Genomic epidemiological analysis of antimicrobial-resistant bacteria with nanopore sequencing.- Rapid and comprehensive identification of nontuberculous mycobacteria.- Long-read single-cell sequencing using scCOLOR-seq.- Unfolding the Bacterial Transcriptome Landscape using Oxford Nanopore Technology Direct RNA Sequencing.- Nanopore Direct RNA Sequencing of Monosome-and Polysome-bound RNA.- RNA modification detection using nanopore direct RNA sequencing and nanoDoc2.
