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This volume provides an understanding of how an immunoassay works, detailing the strengths, weaknesses, pitfalls. Chapters guide readers on how and when to appropriately utilize this powerful tool, examples of where the ELISA or similar immunoassay formats are currently being used, and newer techniques that may have a significant impact on future applications. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge, ELISA: Methods and Protocols is a valuable resource for both novice and expert scientists in this developing field.
List of contents
Enzyme-linked Immunosorbent Assay: Types and Applications.- ELISA Essentials: surfaces, antibodies, enzymes and substrates.- Antibody Immobilization.- Screening for Antibody Specificity and Sensitivity with ELISA.- Effective blocking and stabilizing methods using synthetic polymer on ELISA.- Gas plasma surface modification for biological assays.- Interference in ELISA.- Determination of Proinflammatory And Anti-Inflammatory Cytokines By Elisa Technique.- Gyrolab immunoassays - Miniaturization, automation and integration into a rapid workflow.- Assessment of Immunologically Potent Carbohydrates.- Lateral Flow Microarray based ELISA for Cytokines.- A unique multiplex ELISA to profile growth factors and cytokines in cerebrospinal fluid.- Well-based Multiplex Food Allergen Colorimetric ELISA.- Mycotoxin Quantification by Competitive ELISA.- Lumit: A Homogeneous Bioluminescent Immunoassay for Detecting Diverse Analytes and Intracellular Protein targets.- ELISA-based Biosensors.
