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This detailed collection serves as a unique and excellent collection of state-of-the-art methods and protocols to interrogate cell migration in a wide variety of different contexts and model organisms, as well as advanced image analysis and quantitative assessment of a diverse array of parameters related to cell migration. The book focuses on the cell biology of cell migration, developmental model systems to assess cell migration during morphogenesis, cell migration in cancers and the tumor micro-environment, as well as blood vessel formation and interactions. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Cell Migration in Three Dimensions provides a solid foundation for scientists of different disciplines to investigate cell migration in biological processes.
Chapters 7, 12, 16, 17, 19, 22, and 24 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
List of contents
Cell Migration in Three Dimensions.- Use of Ecto-Tagged Integrins to Monitor Integrin Exocytosis and Endocytosis.- Probing the ER-Focal Adhesion Link during Cell Migration.- Mapping the Localization of Proteins within Filopodia Using FiloMap.- Extended Methods for 2D Confinement.- Visualization of Exosome Release and Uptake during Cell Migration Using the Live Imaging Reporter pHluorin_M153R-CD63.- Approaches to Determine Nuclear Shape in Cells during Migration through Collagen Matrices.- Dissecting Collective Cell Behavior in Migrating Testis Myotubes in Drosophila.- Whole Mount In Situ Hybridization for Detection of Migrating Zebrafish Endodermal Cells.- Live Imaging Analysis of Epithelial Zippering during Mouse Neural Tube Closure.- Time-Lapse Imaging and Morphometric Analysis of Tracheal Development in Drosophila.- A Guide Toward Multi-Scale and Quantitative Branching Analysis in the Mammary Gland.- Analysis of Integrin-Dependent Melanoblast Migration during Development.- Invadopodia Methods: Detection of Invadopodia Formation and Activity in Cancer Cells Using Reconstituted 2D and 3D Collagen-Based Matrices.- Analysis of Energy-Driven Leader-Follower Hierarchy during Collective Cancer Cell Invasion.- Visualizing and Quantifying mRNA Localization at the Invasive Front of 3D Cancer Spheroids.- Multimodal Techniques to Study Tumor Growth, Basement Membrane Breaching, and Invasion in 3D Matrices.- Analysis of Collective Migration Patterns within Tumors.- An In Vivo Model to Study Cell Migration in XYZ-T Dimension Followed by Whole-Mount Re-Evaluation.- Angiogenesis Invasion Assay to Study Endothelial Cell Invasion and Sprouting Behavior.- Live-Cell Labeling and Artificial Intelligence Approaches for High-Resolution XYZT Imaging of Cytoskeletal Dynamics during Collective Cell Migration.- Analysis of mRNA Subcellular Distribution in Collective Cell Migration.- A Bio-Engineered Model for Studying Vascular-Pericyte Interactions of the Placenta.- Analysis of Vascular Morphogenesis in Zebrafish.- Analysis of Monocyte Recruitment during Inflammation by Intravital Imaging.
