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This detailed volume explores the development of technologies and protocols that are currently being used to understand the nature and activities of the plant cytoskeleton. A focus for many of the chapters is on sample preparation, as the quality of plant organ/tissue preparation, from single to multicellular samples, determines the quality of the data. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, The Plant Cytoskeleton: Methods and Protocols serves as an ideal guide for researchers interested in or starting to be interested in plant cell and molecular biology research.
List of contents
Quantification of Microtubule Bundling Activity of MAPs Using TIRF Microscopy.- Actin: Static and Dynamic Studies.- 3D Visualization of Microtubules in Epidermal Pavement Cells.- Quantitative Analysis of Microtubule Organization in Leaf Epidermis Pavement Cells.- Single Cell Confinement Methods to Study Plant Cytoskeleton.- Documentation of Microtubule Collisions with Myosin VIII ATM1 Containing Membrane-Associated Structures.- Imaging the Plant Cytoskeleton by High-Pressure Freezing and Electron Tomography.- Confocal Microscopic Assays of Mitotically Active Proteins in an Agrobacterial Infiltration-Based, Cell Division-Enabled Leaf System of Tobacco (Nicotiana benthamiana).- Assessment of Spindle Shape Control by Spindle Poleward Flux Measurements and FRAP Bulk Analysis.- Expansion Microscopy of Plant Cells (PlantExM).- Microfluidic Device for High-Resolution Cytoskeleton Imaging and Wash Out Assays in Physcomitrium (Physcomitrella) patens.- Using Spinning Disk Microscopy to Observe the Mitotic and Cytokinetic Apparatus in Physcomitrium patens.- Gaining Insight into Large Gene Families with the Aid of Bioinformatic Tools.- Cell-to-Cell Connectivity Assays for the Analysis of Cytoskeletal and Other Regulators of Plasmodesmata.- Studying Nuclear Dynamics in Response to Actin Disruption In Planta.- Cytoskeleton Remodelling in Arabidopsis Stigmatic Cells Following Pollination.- Investigation of ROP GTPase Activity and Cytoskeleton Dynamics during Tip Growth in Root Hairs and Pollen Tubes.- Functional Analysis of Phospholipid Signaling and Actin Dynamics: The Use of Apical Growing Tobacco Pollen Tubes in a Case Study.- Microtubule Reorganization during ABA-Induced Stomatal Closure in Arabidopsis.- Imaging of Cortical Microtubules in Plants under Salt Stress.- Analysis of Actin Array Rearrangement during the Plant Response to Bacterial Stimuli.- Live-Cell Imaging of Cytoskeletal Responses and Trafficking during Fungal Elicitation.- Visualization and Quantification of the Dynamics of Actin Filaments in Arabidopsis Pollen Tubes.- Non-Invasive Long-Term Imaging of the Cytoskeleton in Arabidopsis Seedlings.- Visualization of Cytoskeleton Organization and Dynamics in Elongating Cotton Fibers by Live-Cell Imaging.- Methods to Visualize and Quantify Cortical Microtubule Arrays in Arabidopsis Conical Cells.- Studying the Organization of the Actin Cytoskeleton in the Multicellular Trichomes of Tomato.- Light Microscopy Technologies and the Plant Cytoskeleton.- Investigating Plant Protein-Protein Interactions Using FRET-FLIM with a Focus on the Actin Cytoskeleton.
