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This second edition provides update and new chapters detailing core and emerging in vitro and in vivo protocols. Chapters guide readers through cellular and molecular biology approaches, in vivo genetic approaches, various "omics"-based strategies, therapeutic strategies, and advanced techniques in the fields of tissue engineering and nanotechnology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, The Tumor Microenvironment: Methods and Protocols, Second Edition is a valuable resource for both novice and expert scientists in this developing field.
List of contents
Imaging Mass Cytometry in Immuno-oncology.- Analyzing the Tumor Immune Microenvironment by Flow Cytometry.- Blood sample processing and banking for functional and molecular analyses.- Quantitatively Assessing the Respiratory Burst in Innate Immune Cells.- Visualizing NETosis using a Novel Neutrophil Extracellular Trap-Specific Marker.- In vitro methods to evaluate macrophage polarization and function in cancer.- In vivo analysis of tumor associated macrophages in the tumor microenvironment.- Assessing the immune regulatory role of metabolites in vitro.- Mouse Models of Obesity To Study The Tumor Immune Microenvironment.- Mouse Models of Obesity To Study The Tumor Immune Microenvironment.- Assessing the efficacy of immune checkpoint inhibitors in pre-clinical tumor models.- Fibroblast isolation from mammary gland tissue and syngeneic murine breast cancer models.- Multiscale label-free imaging of fibrillar collagen in the tumor microenvironment.- In situ measurement of intra-tumoral tissue rigidity.- Extracellular matrix glycation and crosslinking in mammary tumor progression.- Proteomic assessment of the murine mammary gland extracellular matrix.- Spheroids as a 3D Model of the Hypoxic Tumor Microenvironment.- Tumor pH imaging using Chemical Exchange Saturation Transfer (CEST)-MRI.- Profiling the epigenetic landscape of the tumor microenvironment using chromatin immunoprecipitation-sequencing.- Analysis of Spatial Molecular Data.- Laser-assisted single-cell labeling and capture.- 3D hydrogel cultures for high-throughput drug discovery.- Intravital microscopy of the metastatic pulmonary environment.- A CRISPR platform for targeted in vivo screens.
