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Conducting studies of natural and man-made air contaminants is a specialized research activity that draws upon skills from a variety of disciplines including toxicology, pharmacology, industrial hygiene, and environmental health. Methods in Inhalation Toxicology describes how knowledge from these disciplines is integrated into the design and conduct of inhalation studies. Each of the vital aspects of conducting these studies is discussed in detail, including the use of animal subjects and the related quality control and ethical considerations, air purification methodology, exposure atmosphere generation and characterization, inhalation exposure systems, and real-time and post-exposure biological assessments. These methods will enable you to conduct inhalation studies, easily adding any specific measures of particular interest to your research.
The book covers techniques needed to:
provide suitable clean air for experiments
correctly generate the particles and gases being studied
select the proper exposure system
monitor the exposure
prepare tissues for study
ensure that animals do not have confounding lung disease
The helpful descriptions and detailed lists of suppliers of preferred equipment and materials will help you to perform valid, reproducible inhalation toxicology studies. Methods in Inhalation Toxicology is a perfect textbook for students in toxicology programs, as well as for scientists who conduct inhalation studies in both academic and industrial environments.
List of contents
Chapter 1. The Basics of the Polymerase Chain Reaction -- /William B. Mattes -- Chapter 2. Analysis of Gene Expression -- /John P. Vanden Heuoel -- Chapter 3. Differential Display PCR -- /J. Christopher Corton -- Chapter 4 . Cloning by PCR -- /John P. Vanden Heuuel -- Chapter 5. Genotype Analysis -- /Douglas A. Bell and Gary Pittman -- Chapter 6 . General Molecular Biology Techniques -- /John P. Vanden Heuvel -- Appendix I - List of Suppliers -- Appendix II - Companies, Online Catalogs, and Internet Resources -- Index.
About the author
Vanden Heuvel, John P.
Summary
A practical guide to the use of polymerase chain reaction (PCR) to help examine, on a molecular and cellular level, how toxic responses are manifested. It explores scientific advances and describes techniques such as differential display cloning and non-PCR based molecular biology methods.
