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Informationen zum Autor Steven Haney is a Senior Research Advisor and Group Leader at Eli Lilly and Company. He edited the book High Content Screening: Science, Techniques, and Applications (Wiley, 2008). Douglas Bowman is an Associate Scientific Fellow at Takeda Pharmaceuticals. Arijit Chakravarty is the Director of Modeling and Simulation (DMPK) at Takeda Pharmaceuticals. Anthony Davies is Center Director, Translational Cell Imaging, Queensland University Of Technology, Queensland, Australia. Caroline Shamu is the Director of the ICCB-Longwood Screening Facility at Harvard Medical School. Klappentext Using a collaborative and interdisciplinary author base with experience in the pharmaceutical industry and academia, this book is a practical resource for high content (HC) techniques.* Instructs readers on the fundamentals of high content screening (HCS) techniques* Focuses on practical and widely-used techniques like image processing and multiparametric assays* Breaks down HCS into individual modules for training and connects them at the end* Includes a tutorial chapter that works through sample HCS assays, glossary, and detailed appendices Zusammenfassung Using a collaborative and interdisciplinary author base with experience in the pharmaceutical industry and academia, this book focuses on the fundamental principles of high content (HC) techniques; emphasizing underlying fields of image processing, microscopy, cell biology, informatics, and data analysis. Inhaltsverzeichnis PREFACE xvii CONTRIBUTORS xix 1 Introduction 1 Steven A. Haney 1.1 The Beginning of High Content Screening, 1 1.2 Six Skill Sets Essential for Running HCS Experiments, 4 1.3 Integrating Skill Sets into a Team, 7 1.4 A Few Words on Experimental Design, 8 1.5 Conclusions, 9 Key Points, 9 Further Reading, 10 References, 10 SECTION I FIRST PRINCIPLES 11 2 Fluorescence and Cell Labeling 13 Anthony Davies and Steven A. Haney 2.1 Introduction, 13 2.2 Anatomy of Fluorescent Probes, Labels, and Dyes, 14 2.3 Stokes' Shift and Biological Fluorophores, 15 2.4 Fluorophore Properties, 16 2.5 Localization of Fluorophores Within Cells, 18 2.6 Multiplexing Fluorescent Reagents, 26 2.7 Specialized Imaging Applications Derived from Complex Properties of Fluorescence, 27 2.8 Conclusions, 30 Key Points, 31 Further Reading, 31 References, 31 3 Microscopy Fundamentals 33 Steven A. Haney, Anthony Davies, and Douglas Bowman 3.1 Introducing HCS Hardware, 33 3.2 Deconstructing Light Microscopy, 37 3.3 Using the Imager to Collect Data, 43 3.4 Conclusions, 45 Key Points, 45 Further Reading, 46 References, 46 4 Image Processing 47 John Bradley, Douglas Bowman, and Arijit Chakravarty 4.1 Overview of Image Processing and Image Analysis in HCS, 47 4.2 What is a Digital Image?, 48 4.3 "Addressing" Pixel Values in Image Analysis Algorithms, 48 4.4 Image Analysis Workflow, 49 4.5 Conclusions, 60 Key Points, 60 Further Reading, 60 References, 60 SECTION II GETTING STARTED 63 5 A General Guide to Selecting and Setting Up a High Content Imaging Platform 65 Craig Furman, Douglas Bowman, Anthony Davies, Caroline Shamu, and Steven A. Haney 5.1 Determining Expectations of the HCS System, 65 5.2 Establishing an HC Platform Acquisition Team, 66 5.3 Basic Hardware Decisions, 67 5.4 Data Generation, Analysis, and Retention, 72 5.5 Installation, 73 5.6 Managing the System, 75 5.7 Setting Up Workflows for Researchers, 77 5.8 Conc...
