Essential Guide to Reading Biomedical Papers - Recognising and Interpreting Best Practice

Informationen zum Autor Dr. Philip David Langton, Senior Lecturer and Academic Director of E-Learning, Department of Physiology, University of Bristol Dr Philip Langton is currently a Senior Lecturer and Academic Director of e-Learning in the Faculty of Medical and Veterinary Sciences at the University of Bristol, UK.?Dr Langton is an advocate for excellence in all forms of learning, teaching and assessment in HE. He is an enthusiastic undergraduate and graduate teacher, contributing to Medical Sciences and Physiological Sciences programmes. Finalist of 'Bioscience Teacher of the Year' for 2011, Dr Langton is passionate about providing an excellent learning experience for students in ways that are stimulating and rewarding for University staff, and disseminating innovative and high-quality learning practices. Klappentext Essential Guide to Reading Biomedical Papers: Recognising and Interpreting Best Practice is an indispensable companion to the biomedical literature. This concise, easy-to-follow text gives an insight into core techniques and practices in biomedical research and how, when and why a technique should be used and presented in the literature. Readers are alerted to common failures and misinterpretations that may evade peer review and are equipped with the judgment necessary to be properly critical of the findings claimed by research articles. This unique book will be an invaluable resource for students, technicians and researchers in all areas of biomedicine.* Allows the reader to develop the necessary skills to properly evaluate research articles* Coverage of over 30 commonly-used techniques in the biomedical sciences* Global approach and application, with contributions from leading experts in diverse fields Zusammenfassung * Allows the reader to develop the necessary skills to properly evaluate research articles * Coverage of over 30 commonly-used techniques in the biomedical sciences * Global approach and application, with contributions from leading experts in diverse fields . Inhaltsverzeichnis List of contributors ix Foreword xi Preface xiii Acknowledgements xvii Introduction xix Section A - Basic principles 1 1 Philosophy of science 3 James Ladyman 2 Ingredients of experimental design 9 Nick Colegrave 3 Statistics: a journey that needs a guide 17 Gordon Drummond Section B - Cell and molecular 27 4 Organ bath pharmacology 29 Emma Robinson 5 Small vessel myography 39 Tomoko Kamishima and John M Quayle 6 Mammalian cell cultures: the example of airway epithelial cell cultures for cystic fibrosis research 49 Scott H Randell 7 Electron microscopy (TEM and SEM) 59 Paul Verkade 8 Fluorescence microscopy 67 Mark Jepson 9 Intracellular 'sharp' microelectrode recording 77 Helena C Parkington and Harold A Coleman 10 Single electrode voltage-clamp (SEVC) 85 Harold A Coleman and Helena C Parkington 11 Patch clamp recording 95 Neil Bannister and Phil Langton 12 Production of antibodies 105 Elek Molnar 13 Immunocytochemistry and immunohistochemistry 117 Elek Molnar 14 Immunoprecipitation (IP) 129 David Bates 15 Immunoblotting (western) 137 Samantha F. Moore, Joshua S. Savage and Ingeborg Hers 16 Applications of green fluorescent protein (GFP) 147 Mark Jepson 17 Fluorescent measurement of ion activity in cells 153 Helen Kennedy 18 Detection of exocytosis - real time 163 Anja Teschemacher 19 Viral vector transgenesis 173 Anja Teschemacher 20 Polymerase chain reaction (PCR) and reverse transcription (RT)-PCR 179 Lucy F. Donaldson 2...

List of contents

List of contributors ix
 
Foreword xi
 
Preface xiii
 
Acknowledgements xvii
 
Introduction xix
 
Section A - Basic principles 1
 
1 Philosophy of science 3
James Ladyman
 
2 Ingredients of experimental design 9
Nick Colegrave
 
3 Statistics: a journey that needs a guide 17
Gordon Drummond
 
Section B - Cell and molecular 27
 
4 Organ bath pharmacology 29
Emma Robinson
 
5 Small vessel myography 39
Tomoko Kamishima and John M Quayle
 
6 Mammalian cell cultures: the example of airway epithelial cell cultures for cystic fibrosis research 49
Scott H Randell
 
7 Electron microscopy (TEM and SEM) 59
Paul Verkade
 
8 Fluorescence microscopy 67
Mark Jepson
 
9 Intracellular 'sharp' microelectrode recording 77
Helena C Parkington and Harold A Coleman
 
10 Single electrode voltage-clamp (SEVC) 85
Harold A Coleman and Helena C Parkington
 
11 Patch clamp recording 95
Neil Bannister and Phil Langton
 
12 Production of antibodies 105
Elek Molnar
 
13 Immunocytochemistry and immunohistochemistry 117
Elek Molnar
 
14 Immunoprecipitation (IP) 129
David Bates
 
15 Immunoblotting (western) 137
Samantha F. Moore, Joshua S. Savage and Ingeborg Hers
 
16 Applications of green fluorescent protein (GFP) 147
Mark Jepson
 
17 Fluorescent measurement of ion activity in cells 153
Helen Kennedy
 
18 Detection of exocytosis - real time 163
Anja Teschemacher
 
19 Viral vector transgenesis 173
Anja Teschemacher
 
20 Polymerase chain reaction (PCR) and reverse transcription (RT)-PCR 179
Lucy F. Donaldson
 
21 In situ hybridisation (ISH) 187
Lucy F. Donaldson
 
22 Methods of interference (antisense, siRNAs and dominant negative mutations) 193
Allison Fulford
 
23 Transcriptome analysis: microarrays 203
Charles Hindmarch
 
24 Experimental proteomics 215
Thierry Le Bihan
 
Section C - In vivo / integrative 229
 
25 Behavioural methodology 231
Emma Robinson
 
26 Genetically modified mouse models 241
Nina Balthasar
 
27 Wireless recording of cardiovascular signals 247
Julian FR Paton and Fiona D McBryde
 
28 Electrical stimulation methods 253
Jon Wakerley
 
29 Extracellular recording 261
Jon Wakerley
 
30 Antidromic identification 271
Jon Wakerley
 
31 Event-triggered averaging, including spike-triggered averaging 279
Richard Apps
 
32 Axonal transport tracing of CNS pathways 285
John Crabtree
 
33 Cardiovascular methods: general considerations for human studies 291
Erica A Wehrwein and Michael J Joyner
 
34 Measuring cardiac output in humans 299
Erica A Wehrwein and Michael J Joyner
 
35 Measuring peripheral blood flow in humans 311
Erica A Wehrwein and Michael J Joyner
 
Index 319

Report

"The book would be of most benefit to new researchers or early career scientists though with some benefits to the more experienced scientist moving into a new discipline." (Microbiology Today, 1 September 2013)
 
"As a guide to how to properly ask questions of life and how to interpret its often-enigmatic answers, this book is a must read. Summing Up: Highly recommended. Upper-division undergraduates, graduate students, researchers/faculty, and professionals." (Choice, 1 August 2013)
 
"Too many recent bioscience graduates lack significant research lab experience. Even research projects can expose them to just a few techniques. Appraisal of research papers in undergraduate courses tends to address the results, but rarely includes a critical evaluation of the researchers methodology. In reality, few bioscientists can claim a working knowledge of more than a handful of the techniques covered in this collection.
 
These shortcomings can be greatly overcome thanks to this book. The contributors are active research scientists of high quality. Each addresses the methods in a critical sense and provide an expert's view of the advantages and pitfalls. There is no equivalent book currently available." Dr David J. Miller on behalf of The Physiological Society
 
"Research questions require the scientist to employ molecular, cell, organism and population approaches, and the reader of scientific literature to carefully consume data from each of those environments. This book provides a clear and concise summary of the research techniques and approaches that generate data. Importantly, the authors identify appropriate uses and cautionary caveats, essential for readers who do not use the technique. This book is essential for accurate and careful interpretation of scientific literature." Professor Robert G. Carroll, Editor Advances in Physiology Education

Product details

• Authors: Philip D. Langton
• Assisted by: Philip D Langton (Editor), Philip D. Langton (Editor), Langton Philip D. (Editor)
• Publisher: Wiley, John and Sons Ltd
• Languages: English
• Product format: Paperback / Softback
• Released: 23.11.2012
• EAN: 9781119959977
• ISBN: 978-1-119-95997-7
• No. of pages: 352
• Subjects: Natural sciences, medicine, IT, technology > Biology > General, dictionaries; Soziologie, Sociology, Life Sciences, Biowissenschaften, Allg. Biowissenschaften, Soziologische Forschungsmethoden, Research Methodologies