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Informationen zum Autor Dr. Philip David Langton, Senior Lecturer and Academic Director of E-Learning, Department of Physiology, University of Bristol Dr Philip Langton is currently a Senior Lecturer and Academic Director of e-Learning in the Faculty of Medical and Veterinary Sciences at the University of Bristol, UK.?Dr Langton is an advocate for excellence in all forms of learning, teaching and assessment in HE. He is an enthusiastic undergraduate and graduate teacher, contributing to Medical Sciences and Physiological Sciences programmes. Finalist of 'Bioscience Teacher of the Year' for 2011, Dr Langton is passionate about providing an excellent learning experience for students in ways that are stimulating and rewarding for University staff, and disseminating innovative and high-quality learning practices. Klappentext Essential Guide to Reading Biomedical Papers: Recognising and Interpreting Best Practice is an indispensable companion to the biomedical literature. This concise, easy-to-follow text gives an insight into core techniques and practices in biomedical research and how, when and why a technique should be used and presented in the literature. Readers are alerted to common failures and misinterpretations that may evade peer review and are equipped with the judgment necessary to be properly critical of the findings claimed by research articles. This unique book will be an invaluable resource for students, technicians and researchers in all areas of biomedicine.* Allows the reader to develop the necessary skills to properly evaluate research articles* Coverage of over 30 commonly-used techniques in the biomedical sciences* Global approach and application, with contributions from leading experts in diverse fields Zusammenfassung * Allows the reader to develop the necessary skills to properly evaluate research articles * Coverage of over 30 commonly-used techniques in the biomedical sciences * Global approach and application, with contributions from leading experts in diverse fields . Inhaltsverzeichnis List of contributors ix Foreword xi Preface xiii Acknowledgements xvii Introduction xix Section A - Basic principles 1 1 Philosophy of science 3 James Ladyman 2 Ingredients of experimental design 9 Nick Colegrave 3 Statistics: a journey that needs a guide 17 Gordon Drummond Section B - Cell and molecular 27 4 Organ bath pharmacology 29 Emma Robinson 5 Small vessel myography 39 Tomoko Kamishima and John M Quayle 6 Mammalian cell cultures: the example of airway epithelial cell cultures for cystic fibrosis research 49 Scott H Randell 7 Electron microscopy (TEM and SEM) 59 Paul Verkade 8 Fluorescence microscopy 67 Mark Jepson 9 Intracellular 'sharp' microelectrode recording 77 Helena C Parkington and Harold A Coleman 10 Single electrode voltage-clamp (SEVC) 85 Harold A Coleman and Helena C Parkington 11 Patch clamp recording 95 Neil Bannister and Phil Langton 12 Production of antibodies 105 Elek Molnar 13 Immunocytochemistry and immunohistochemistry 117 Elek Molnar 14 Immunoprecipitation (IP) 129 David Bates 15 Immunoblotting (western) 137 Samantha F. Moore, Joshua S. Savage and Ingeborg Hers 16 Applications of green fluorescent protein (GFP) 147 Mark Jepson 17 Fluorescent measurement of ion activity in cells 153 Helen Kennedy 18 Detection of exocytosis - real time 163 Anja Teschemacher 19 Viral vector transgenesis 173 Anja Teschemacher 20 Polymerase chain reaction (PCR) and reverse transcription (RT)-PCR 179 Lucy F. Donaldson 2...
