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Informationen zum Autor Virginia Litwin is a Principal Scientist at Covance Central Laboratory Services. After numerous years in the pharmaceutical/biotechnology industry, she has become a leader in the application and validation of flow cytometric methods in drug discovery and development. She founded the Flow Cytometry Subcommittee of the Ligand Binding Assay Bioanalytical Focus Group within the American Association of Pharmaceutical Scientists (AAPS). She is an active contributor to the International Clinical Cytometry Society (ICCS) and the International Society for Analytical Cytology (ISAC). She received her bachelor's from the University of California and her doctorate from the University of Iowa. Philip Marder was internationally distinguished for his leadership in the application of flow cytometry in early drug discovery and development. He was a key contributor to the scientific community as an active member of ISAC and AAPS. After receiving both his bachelor's and master's degrees from The Ohio State University, he had a distinguished thirty year career at Eli Lilly and Company. Klappentext Overview of Flow Cytometry in Drug Discovery and Development In this book, the unique application of flow cytometry in pharmaceutical discovery and development is presented. A basic explanation of the technology precedes a comprehensive review of specific examples of how the technology is utilized in drug development. Readers of this book will learn how flow cytometry's use of multiparametric, high-speed measurements and manipulations of single cells adds valuable information and efficiencies for the discovery and development of new, better, and more affordable pharmaceuticals. It discusses validation and regulatory compliance issues as well as the use of cytometry in monitoring clinical diseases and evaluating therapies. Divided into four parts, the book: Includes two introductory chapters, one on flow cytometry and one on biomarkers, as well as a chapter on recent advances in flow cytometry Focuses on the unique challenges and added benefits associated with the use of flow cytometry in the drug development process Presents an in-depth discussion of validation considerations and regulatory compliance issues associated with drug development Ends with two chapters addressing important future directions in the field of flow cytometry Written by thought-leaders from academia and industry as well as flow cytometry users from the pharmaceutical sector, this comprehensive and straightforward text helps pharmaceutical researchers understand the potential of flow cytometry and, in turn, allows flow cytometrists to understand the needs of the pharmaceutical industry. Zusammenfassung The unique application of flow cytometry in drug discovery and development is the focus of this book. It covers flow cytometry and biomarkers, information on recent advances in flow cytometry, and presents the unique challenges and added benefits associated with the use of flow cytometry in the drug development process. Inhaltsverzeichnis Preface xi Foreword xiii Acknowledgments xv Contributors xvii Part I Introduction 1 Philip Marder and Virginia Litwin 1 Introduction to Flow Cytometry 3 Elizabeth Raveche, Fatima Abbasi, Yao Yuan, Erica Salerno, Siddha Kasar, and Gerald E. Marti 2 Recent Advances in Flow Cytometry: Platforms, Tools, and Challenges for Data Analysis 23 Paul J. Smith, Roy Edward, and Rachel J. Errington 3 Introduction to Biomarkers 55 Ole Vesterqvist and Manjula P. Reddy Part II Flow Cytometry In The Drug Development Process 69 Virginia Litwin and Philip Marder 4 HTS Flow Cytometry, Small-Molecule Discovery, and the NIH Molecular Libraries Initiative 71 Larry A. Sklar and Br...
