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Informationen zum Autor Ge Wu, PhD, is Director of Assay Development at Iris International. He obtained his doctorate in biochemistry from UCLA, performed postdoctoral work at Harvard Medical School, and was a member of the faculty at Cornell University Medical College. Prior to joining Iris International, he worked at Merck & Co., Caliper Technologies, Amphora Discovery, and FivePrime Therapeutics. He is the founding Director of Assay Development and Screening at FivePrime Therapeutics. Dr. Wu is a leading figure in assay development and screening in biopharmaceutical discovery using primary cell assay and the author of an acclaimed review in this subject. Klappentext The first comprehensive, integrated treatment of the subject, Assay Development: Fundamentals and Practices covers the essentials and techniques involved in carrying out an assay project in either a biotechnology/drug discovery setting or a platform setting. After discussing the basics, it explains the treatment of assays for purified protein, cell-based assays, and high-throughput screening. It includes good practices and case studies. This is a premier resource for biotechnologists, analytical chemists, biochemists, medicinal chemists, toxicologists, and others, and an excellent text for professors preparing students to enter the biotechnology and pharmaceutical industries. Zusammenfassung The first comprehensive, integrated treatment of the subject, Assay Development: Fundamentals and Practices covers the essentials and techniques involved in carrying out an assay project in either a biotechnology/drug discovery setting or a platform setting. Inhaltsverzeichnis Preface. Chapter 1 Introduction to assay development. 1.1 Assay and Bioassay. 1.2 Drug discovery process and the role of assays in the process. 1.3 Bioassay development. 1.4 Bioassay Classifications. Chapter 2 Measurement and Instrumentation. 2.1 Measurement and perturbation.. 2.2 Common instrumental methods and instrument components. 2.3 Molecular absorption measurements. 2.4 Molecular luminescence measurements. 2.5 Luminescence lifetime measurement and time-resolved fluorescence (TRF) measurement.. 2.6 Fluorescence resonance energy transfer (FRET) and TR-FRET. 2.7 Fluorescence quenching. 2.8 Fluorescence polarization. 2.9 Radioactivity measurement. 2.10 Evaluating and selecting an instrumental method for bioassay. Chapter 3 Fundamental principles of assay with isolated proteins. 3.1 Chemical potential, equilibrium, and kinetics. 3.2 Protein binding studies at equilibrium. 3.3 Kinetic studies of protein binding process. 3.4 Enzyme kinetics. 3.5 Inhibition of protein function. 3.6 Assay development with isolated proteins. Chapter 4 Separation-based techniques in bioassays. 4.1 Washing to remove impurity on solid support. 4.2 Organic extraction of hydrophobic molecules. 4.3 Centrifugation to remove dense particles. 4.4 Membrane filtration. 4.5 Liquid chromatography. 4.6 Electrophoresis. Chapter 5 General protein binding assay formats. 5.1 Equilibrium dialysis. 5.2 Competitive binding assays with radioactive or other labeled ligands. 5.3 Application of SPATM and FlashplateTM in binding studies. 5.4 Application of FP assays in binding studies. 5.5 Application of FRET assays in binding studies. 5.6 Application of ELISA in binding studies. 5.7 Surface plasmon resonance (SPR) technology and its application in binding studies. 5.8 Application of label-free technologies in binding studies. Chapter 6 Functional assays with isolated proteases. 6.1 Introduction to proteases and their substrates. 6.2 The function of proteases and their role drug discovery. 6.3 Protease assays...
