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Informationen zum Autor MARCO MASCINI , PhD, is a Full Professor of Analytical Chemistry in the Department of Chemistry at the University of Firenze, Italy. He has been one of the pioneers of biosensor technology. His research interests are related to electrochemical, piezoelectric, and optical biosensors. He pioneered several practical applications of these devices in industrial prototypes for use in bioanalysis, medicine, the environment, and food quality and safety. Some of these devices are commercialized today. Dr. Mascini is the author of more than 300 publications and has taken part in several European Commission projects and steering committees. Klappentext Explore technologies and methods using aptamers in different applicationsThis book details bioanalytical technologies and methods that have been developed using aptamers in analytical, medical, environmental, and food science applications. Because aptamers have some advantages over their rival antibodies, this is a rapidly emerging field with vast potential in varied disciplines. After an introduction to aptamers, aptamer targets, and their general uses, Aptamers in Bioanalysis:* Discusses different applications, with particular attention to the comparison between aptamer-based biosensors and methods versus the corresponding immunosensors* Includes examples of aptamer-based diagnostic techniques such as whole-cell protein profiling (proteomics) and medical diagnostics for the distinction of diseased versus healthy states* Includes chapters written by leading experts in their fieldsWith an overview of proven bioanalytical technologies and methods and numerous references for further study, this is a core reference for analytical chemists, electrochemists, pharmaceutical/medicinal chemists, and biotechnologists. Zusammenfassung This is the first book to detail bioanalytical technologies and methods that have been developed using aptamers in analytical, medical, environmental, and food science applications. Inhaltsverzeichnis PREFACE. CONTRIBUTORS. I INTRODUCTION. 1 APTAMERS: LIGANDS FOR ALL REASONS ( Jean-Jacques Toulme , Jean-Pierre Daguer, and Eric Dausse ). 1.1 Introduction. 1.2 The Power of Selection and Aptamer Refinement. 1.3 The Chemistry Drives the Shape. 1.4 Aptaregulators. 1.5 Aptasensors. 1.6 Prospects. References. 2 SELEX AND ITS RECENT OPTIMIZATIONS ( Beate Strehlitz and Regina Stoltenburg ). 2.1 Introduction. 2.2 Aptamers and Their Selection by SELEX. 2.3 Modifications of SELEX Technology. 2.4 Advantages and Limitations of Aptamers and Their Selection Technology. 2.5 Applications of Aptamers Being Developed for the Market. 2.6 Future Perspectives. References. II BIOSENSORS. 3 ELECTROCHEMICAL APTASENSORS ( Itamar Willner and Maya Zayats ). 3.1 Introduction. 3.2 Electrochemical Aptasensor Based on Redox-Active Aptamer Monolayers Linked to Electrodes. 3.3 Enzyme-Based Amplified Electrochemical Aptasensors. 3.4 Amplified Electrochemical Aptasensors Based on Nanoparticles. 3.5 Label-Free Electrochemical Aptasensors. 3.6 Field-Effect Transistor-Based Aptasensors. 3.7 Conclusions and Perspectives. References. 4 APTAMERS: HYBRIDS BETWEEN NATURE AND TECHNOLOGY ( Moritz K. Beissenhirtz, Eik Leupold, Walter Stocklein, Ulla Wollenberger, Oliver Panke, Fred Lisdat, and Frieder W. Scheller ). 4.1 Introduction. 4.2 Specific Features of Aptamers. 4.3 Electrochemical Detection of Nucleic Acids. 4.4 Cytochrome c Binding by Aptamers. 4.5 DNA Machines and Aptamers. References. 5 DETECTION OF PROTEIN-APTAMER INTERACTIONS BY MEANS OF ELECTROCHEMICAL INDICATORS AND TRANSVERSE SHEAR MODE METHOD ...
