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This second edition provides state-of-the-art and novel methods on antibiotic isolation and purification, identification of antimicrobial killing mechanisms, as well as methods for the analysis and detection of microbial responses and adaptation strategies. Antibiotics: Methods and Protocols, Second Edition, guides readers through updated and entirely new chapters on production and design, mode of action, and response and resistance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Antibiotics: Methods and Protocols, Second Edition aims to inspire scientific work in the exciting field of antibiotic research.
List of contents
Antibiotics - precious goods in changing times.- A Whole-Cell Assay for Detection of Antibacterial Activity in Actinomycetes Culture Supernatants.- Sampling of Human Microbiomes to Screen for Antibiotic-Producing Commensals.- Production of Antimicrobial Compounds by Homologous and Heterologous Expression.- Isolation and Purification of Natural Products from Microbial Cultures.- Structure Elucidation of Antibiotics.- Computer-Aided Drug Design - An update.- Cytotoxicity Assays as Predictors of the Safety and Efficacy of Antimicrobial Agents.- Microscopy-based Multiwell Assay to Characterize Disturbed Bacterial Morphogenesis Upon Antibiotic Action.- Expansion Microscopy of Bacillus subtilis.- Tracking global and local changes in membrane fluidity through fluorescence spectroscopy and microscopy.- Quantitative analysis of microscopy data to evaluate bacterial responses to antibiotic treatment.- Application of a Bacillussubtilis whole-cell biosensor (PliaI-lux) for the identification of cell wall active antibacterial compounds.- Determination of Bacterial Membrane Impairment by Antimicrobial Agents.- A colorimetric assay to identify and characterize bacterial primase inhibitors.- Cell-based fluorescent screen amenable to HTS to Identify Inhibitors of Bacterial Translation Initiation.- Bacterial two component systems: Overexpression, purification - in vitro and in vivo inhibitor screens.- Sample preparation for mass-spectrometry based absolute quantification of bacterial proteins in antibiotic stress research.- Elemental analysis for the characterization of antimicrobial effects.- label-free quantitation of ribosomal proteins from bacillus subtilis for antibiotic research.- Functional Metagenomics to Study Antibiotic Resistance.
