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This volume provides basic insight and protocols relating to endophytic microbes. Chapter are divided into five major sections detailing basic isolation, bioactive metabolites production. endophytism, isolation and identification of endophytes, bioactive potentials, and screening of metabolites.
Authoritative and cutting-edge, Endophytic Microbes: Isolation, Identification, and Bioactive Potentials aims to provide comprehensive and accessible methods to undergraduate, graduate, and established scientist.
List of contents
Isolation of endophytic bacteria from leaves, stems or roots.- Heat-treatment Method for Isolation of Bacillus spp. from Plant Tissues.- Isolation and detection of endophytic Streptomyces from Actinorhizal Casuarina root nodules.- Isolation of endophytic fungi from stems, leaves or roots.- Detection of endophytes by Cultivation method.- Detection of Endophytes by Visualization Methods.- Detection of endophytes by Immunological methods.- Detection of Endophytes by Electron Microscope.- Detection of Endophytes by Reactive Oxygen Staining.- Detection of Endophytes by Electron Microscope.- Identification of fungal endophytes by ITS rDNA technique.- Identification of Fungal Endophytes by 28srDNATechnique.- Detection of endophytes by molecular fingerprinting techniques.- Metagenomics and Pyrosequencing Approach.- Cultivation-based studies of endophytic fungi in plants.- Microscopy Based Studies.- Surrogate host test.- Hood and Shew staining.- Screening of endophytes for plant growth promoting metabolites.- Screening of endophytes for biocontrol properties.- Screening of Endophytes for Biocatalytic Tools.- Screening of Endophytes for Biosurfactant Production.- Screening of Endophytes for Antibacterial Properties.- Screening of endophytes for Nematicidal activities.- Screening of Endophytes for Virucidal Activity.- Screening of Endophytes for Fungicidal Activities.- Screening of endophytes for anti-cancer compounds.
