Read more
This detailed volume spotlights methods to investigate a variety of virus-host interactions in humans, other mammals, fish, or insects. It explores viruses such as white spot syndrome virus (WSSV), honeybee viruses, Nipah virus, EBV, SVCV, HSV-1, HIV-1, A H1N1, and SARS-CoV-2, as well as applications of techniques such as qPCR, serum antibody responses, 4C analysis, cell membrane fusion, biosensors, computational modelling, quantitative proteomics, and other genetic tools to decipher those viral infections and interactions. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Virus-Host Interactions: Methods and Protocols serves as a valuable resource for researchers both in academia and in the biosciences industry who are engaged in the search for a better understanding of threatening virus-hosts interactions, virus detection, their characterization, and ultimately their taming and control.
List of contents
Genetic Tools for Studying the Roles of Sphingolipids in Viral Infections.- Detection of Serum Antibody Responses in Nipah Virus-Infected Pigs.- Computational Integration of HSV-1 Multi-Omics Data.- A Cell Membrane Fusion Assay for the Fish Pathogen Spring Viremia of Carp Virus (SVCV).- Viral Quantification in Bee Samples Using Synthetic DNA Sequences with Real Time PCR (qPCR).- Inhibition of White Spot Syndrome Virus (WSSV) in Pacific White Shrimp (Litopenaeus vannamei) Using Polyamine-Modified Carbon Quantum Dots.- Computational Modeling of IN-CTD/TAR Complex to Elucidate Additional Strategies to Inhibit HIV-1 Replication.- Protein-Protein and Protein-RNA Interaction Assays to Determine Similarity of INI1/SMARCB1 and TAR RNA in Binding to HIV-1 Integrase.- 4C Analysis of EBV-Host DNA Interactome.- Detection Methods for H1N1 Virus.- Detection of SARS-CoV-2 in Human Breast Milk.- The Rhizopus Holobiont: A Model to Decipher Fungal-Bacterial-Viral Symbioses.- Combining Metabolic Pulse Labeling and Quantitative Proteomics to Monitor Protein Synthesis upon Viral Infection.- Three-Dimensional Human Brain Organoids to Model HIV-1 Neuropathogenesis.- Characterization of SARS-CoV-2 Glycoprotein Using a Quantitative Cell-Cell Fusion System.- A Lentiviral Pseudotype System to Characterize SARS-CoV-2 Glycoprotein.
