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Klappentext Never before has it been so critical for lab workers to possess the proper tools and methodologies necessary to determine the structure! function! and expression of the corresponding proteins encoded in the genome. Mulhardt's Molecular Biology and Genomics helps aid in this daunting task by providing the reader with tips and tricks for more successful lab experiments. This strategic lab guide explores the current methodological variety of molecular biology and genomics in a simple manner! addressing the assets and drawbacks as well as critical points. It also provides short and precise summaries of routine procedures as well as listings of the advantages and disadvantages of alternative methods. * Shows how to avoid experimental dead ends and develops an instinct for the right experiment at the right time* Includes a handy Career Guide for researchers in the field* Contains more than 100 extensive figures and tables Zusammenfassung Exploring the methodological variety of molecular biology and genomics! this book addresses the assets and drawbacks as well as critical points. It provides summaries of routine procedures as well as listings of the advantages and disadvantages of alternative methods. It also shows how to avoid experimental dead ends.
List of contents
Chapter 1: What Is Molecular Biology?
Chapter 2: Fundamental Methods
Chapter 3: The Tools
Chapter 4: The Polymerase Chain Reaction
Chapter 5: RNA
Chapter 6: Cloning DNA Fragments
Chapter 7: Hybridization: How to Track Down DNA
Chapter 8: DNA Analysis
Chapter 9: Investigating the Function of DNA Sequences
Chapter 10: Using Computers
Chapter 11: Suggestions for Career Planning: The Machiavelli Short Course for Young Researchers
Chapter 12: Concluding Thoughts
Appendix 1
Appendix 2
Index
Report
Praise for the German edition:
"This lab book illustrates ways out of experimental dead ends and teaches a feeling for doing the right experiment at the right time." - SWISS LABORATORY MAGAZINE
"With innumerable tips to complete basic knowledge, Mulhardt paves the cumbersome ways from nucleic acids to their translation to gene expression, gene therapy and more." - LABORATORY JOURNAL
"It's no miracle that this book has had a major sales impact." - FOOD AND BIOTECHNOLOGY
"The book reveals the laboratory secrets that are usually learned the hard way, like how not to let agarose boil over in the microwave. Besides protocols and buffer recipes, the book gives advice for setting realisitc goals and eventually becoming an independent scientist , including these encouraging words: 'The experiment will be successful, as long as you are strong enough to put up with six months of the deepest depressioin.' I wish I had this book when I started graduate school."
-Wendy Chao, in THE SCIENTIST, April 1, 2007
