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This volume details applications in molecular biological techniques and focuses on applications to determine the involvement of glycans in virus interactions. Chapters guide readers through glycan analysis, glycan distribution analysis, glycan and lectin microarray, preparation of recombinant viral domain protein, reverse genetics and receptor binding, virus-host interactions and receptor binding, and sialidase: assays and inhibitors. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Glycovirology: Methods and Protocols aims to be an essential resource for researchers who wish to learn more about glycovirology.
List of contents
Comprehensive cellular glycan profiling of glycoproteins and glycosphingolipids by glycoblotting and BEP methods.- High-performance liquid chromatography mapping method for the structural analysis of N-glycans at the molecular, cellular, and tissue levels.- Detection of sialic acids on the cell surface using flow cytometry.- Lectin Staining to Detect Human and Avian influenza Virus Receptors in the Airway of Nonhuman Primates.- Preparation of Glycan Arrays Using Glycopeptides Derived from Biomaterials.- Glycan profiling of viral glycoproteins with the lectin microarray.- Preparation of recombinant PA endonuclease domain protein of influenza A virus and its application for glycobiology research.- Receptor-binding specificity of influenza viruses.-Functional analysis of sulphatide in influenza A virus infection and replication.- Quantification of receptor-association, -dissociation and NA-dependent motility of influenza A particles by biolayer interferometry.- Receptor-binding assay for avian influenza viruses.- Characterization of influenza virus binding to receptors on isolated cell membranes.- Characterization of human parainfluenza virus receptor using terminal sialic acid linkage-modified cells.- Solid-phase binding assay for ganglioside-binding of human respiroviruses.- Evaluation of the glycan-binding and esterase activities of hemagglutinin-esterase-fusion glycoprotein from influenza D virus.- Roles of glycans and non-glycans on the epithelium and in the immune system in H1-H18 influenza A virus infections.- Roles of sialyl glycans in HCoV-OC43, HCoV-HKU1, MERS-CoV and SARS-CoV-2 infections.- Enzymatic substrates and fluorescence imaging of influenza virus sialidase.- Assays for determining the sialidase activity of influenza viruses and monitoring influenza virus susceptibility to neuraminidase inhibitors.- Synthesis and Neuraminidase Inhibitory Activity of Sialic Acid Analogues with Fluoro, Phosphono and Sulfo Functionalities.- Influenza A virus neuraminidase inhibitors.
