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This detailed volume provides an updated overview of current uses of peptide microarray technology, showcasing consolidated applications while highlighting some of the most intriguing novelties and emerging fields of use. The methodologies within this collection are of considerable value for both advanced users and new-comers in the peptide microarray arena, as the renowned contributors describe full coverage of the aspects related to their workflows, from microchip manufacturing to advanced analytical applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Peptide Microarrays: Methods and Protocols, Third Edition aims to make the use of peptide microarrays more and moreaccessible while stimulating further developments to sustain exciting discoveries in the biochemistry and medicine realms.
List of contents
Applications of Peptide Microarrays in Autoantibody, Infection, and Cancer Detection.- Peptide Microarrays for Studying Autoantibodies in Neurological Disease.- A Bi-Functional Polymeric Coating for the Co-Immobilization of Proteins and Peptides on Microarrays Substrates.- Manufacturing of Peptide Microarrays Based on Catalyst-Free Click Chemistry.- Hybrid Peptide-Agarose Hydrogels for 3D Immunoassays.- Low-Cost Peptide Microarrays for Mapping Continuous Antibody Epitopes.- Fast Protocols for Characterizing Antibody-Peptide Binding.- Peptides and Anti-Peptide Antibodies for Small and Medium Scale Peptide and Anti-Peptide Affinity Microarrays.- One-Shot Generation of Epitope-Directed Monoclonal Antibodies to Multiple Non-Overlapping Targets: Peptide Selection, Antigen Preparation, and Epitope Mapping.- Peptide Microarray-Based Protein Interaction Studies Across Affinity Ranges: Enzyme Stalling, Cross-Linking, Depletion, and Neutralization.- Profiling of Multiple Matrix Metalloproteinases Activities in the Progression of Osteosarcoma by Peptide Microarray-Based Fluorescence Assay on Polymer Brush Coated Zinc Oxide Nanorod Substrate.- Peptide Array-Based In Situ Fluorescence Assay for Profiling Multiple Matrix Metalloproteinases Activities.- Peptide-Based Sensor and Microfluidic Platform for IgG Antibody Detection by Differential Impedance Sensing.- Peptide Microarrays for Flavivirus Diagnosis.- Epitope Mapping on Microarrays, Highlight a Sequence on the N Protein with Strong Immune-Response in SARS-CoV-2 Patients.- IgE and IgG4 Epitope Mapping of Food Allergens with a Peptide Microarray Immunoassay.- Extracellular Vesicles Molecular Profiling for Diagnostic Purposes: An Application of Phage-Display Technology.- Membrane-Sensing Peptides for Extracellular Vesicles Analysis.
